Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5840
Title: Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis.
Authors: Abánades, Daniel Ruiz
Arruda, Leonardo Vicentini
Arruda, Elaine S
Pinto, José Roberto A. S
Palma, Mário Sérgio
Aquino, Dorlene Maria Cardoso de
Caldas, Arlene de Jesus Mendes
Soto, Manuel
Barral, Aldina Maria Prado
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
University of São Paulo State. Institute of Biosciences of Rio Claro. Rio Claro, São Paulo, Brasil
University of São Paulo State. Institute of Biosciences of Rio Claro. Rio Claro, São Paulo, Brasil
Universidade Federal do Maranhão. Departamento de Enfermagem. São Luis, Maranhão, Brasil
Universidade Federal do Maranhão. Departamento de Enfermagem. São Luis, Maranhão, Brasil
Universidad Autónoma de Madrid. Departamento de Biologia Molecular. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Abstract: Protection and recovery from visceral leishmaniasis (VL) have been associated with cell-mediated immune (CMI) responses, whereas no protective role has been attributed to humoral responses against specific parasitic antigens. In this report, we compared carefully selected groups of individuals with distinct responses to Leishmania chagasi to explore antigen-recognizing IgG present in resistant individuals. METHODOLOGY AND PRINCIPAL FINDINGS: VL patients with negative delayed-type hypersensitivity (DTH) were classified into the susceptible group. Individuals who had recovered from VL and converted to a DTH+ response, as well as asymptomatic infected individuals (DTH+), were categorized into the resistant group. Sera from these groups were used to detect antigens from L. chagasi by conventional and 2D Western blot assays. Despite an overall reduction in the reactivity of several proteins after DTH conversion, a specific group of proteins (approximately 110-130 kDa) consistently reacted with sera from DTH converters. Other antigens that specifically reacted with sera from DTH+ individuals were isolated and tandem mass spectrometry followed by database query with the protein search engine MASCO were used to identify antigens. The serological properties of recombinant version of the selected antigens were tested by ELISA. Sera from asymptomatic infected people (DTH+) reacted more strongly with a mixture of selected recombinant antigens than with total soluble Leishmania antigen (SLA), with less cross-reactivity against Chagas disease patients' sera. SIGNIFICANCE: Our results are the first evidence of leishmania proteins that are specifically recognized by sera from individuals who are putatively resistant to VL. In addition, these data highlight the possibility of using specific proteins in serological tests for the identification of asymptomatic infected individuals
DeCS: Antígenos de Protozoários/imunologia
Epitopos Imunodominantes/imunologia
Leishmania/imunologia
Leishmaniose Visceral/imunologia
Leishmaniose Visceral/prevenção & controle
Adolescente
Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/química
Western Blotting
Criança
Pré-Escolar
Biologia Computacional
Ensaio de Imunoadsorção Enzimática
Feminino
Epitopos Imunodominantes/química
Imunoglobulina G/sangue
Lactente
Leishmania/química
Peso Molecular
Espectrometria de Massas em Tandem
Issue Date: 2012
Citation: ABÁNADES, D.R. et al.Immunodominant antigens of Leishmania chagasi associated with protection against human visceral leishmaniasis. PLoS Neglected Tropical Diseases, v. 6, n. 6, p. e1687, 2012.
DOI: 10.1371/journal.pntd.0001687
ISSN: 1935-2735
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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