Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5844
Title: Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity
Authors: Costa, Jaqueline França
Wanderley, João Luiz Mendes
Deolindo, Poliana
Zarattini, Jessica B
Costa, Jackson Mauricio Lopes
Soong, Lynn
Barcinski, Marcello André
Barral, Aldina Maria Prado
Borges, Valeria de Matos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Rio de Janeiro. Pólo Universitário Macaé. Rio de Janeiro, RJ, Brasil
Instituto Nacional do Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil
Instituto Nacional do Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
University of Texas Medical Branch. Departments of Microbiology & Immunology and Pathology. Galveston, Texas, United States of America
Instituto Nacional do Câncer. Divisão de Medicina Experimental. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Diffuse cutaneous leishmaniasis (DCL) is a rare clinical manifestation of leishmaniasis, characterized by an inefficient parasite-specific cellular response and heavily parasitized macrophages. In Brazil, Leishmania (Leishmania) amazonensis is the main species involved in DCL cases. In the experimental model, recognition of phosphatidylserine (PS) molecules exposed on the surface of amastigotes forms of L. amazonensis inhibits the inflammatory response of infected macrophages as a strategy to evade the host immune surveillance. In this study, we examined whether PS exposure on L. amazonensis isolates from DCL patients operated as a parasite pathogenic factor and as a putative suppression mechanism of immune response during the infection. Peritoneal macrophages from F1 mice (BALB/c×C57BL/6) were infected with different L. amazonensis isolates from patients with localized cutaneous leishmaniasis (LCL) or DCL. DCL isolates showed higher PS exposure than their counterparts from LCL patients. In addition, PS exposure was positively correlated with clinical parameters of the human infection (number of lesions and time of disease) and with characteristics of the experimental infection (macrophage infection and anti-inflammatory cytokine induction). Furthermore, parasites isolated from DCL patients displayed an increased area in parasitophorous vacuoles (PV) when compared to those isolated from LCL patients. Thus, this study shows for the first time that a parasite factor (exposed PS) might be associated with parasite survival/persistence in macrophages and lesion exacerbation during the course of DCL, providing new insights regarding pathogenic mechanism in this rare chronic disease.
DeCS: Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose Tegumentar Difusa/parasitologia
Fosfatidilserinas/farmacologia
Animais
Doença Crônica
Citocinas/biossíntese
Relação Dose-Resposta a Droga
Humanos
Tolerância Imunológica/efeitos de drogas
Leishmania/isolamento & purificação
Leishmaniose Tegumentar Difusa/imunologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Macrófagos/parasitologia
Camundongos
Issue Date: 2012
Citation: FRANÇA-COSTA, J. et al. Exposure of phosphatidylserine on Leishmania amazonensis isolates is associated with diffuse cutaneous leishmaniasis and parasite infectivity. PLoS One, v. 7, n. 5, p. e36595, 2012.
DOI: 10.1371/journal.pone.0036595
ISSN: 1932-6203
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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