Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5849
Title: Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009).
Authors: Andrade, Sonia Gumes
Campos, Rozalia Mendes Figueira
Steindel, Mario
Guerreiro, Marcos Lázaro da Silva
Magalhães, Juracy Barbosa
Almeida, Marcio Cerqueira de
Reis, Joice Neves
Santos, Viviane Corrêa
Valadares, Helder Magno Silva
Reis, Mitermayer Galvão dos
Macedo, Andréa Mara
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia. Florianópolis, SC, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
Abstract: We examined strains of Trypanosoma cruzi isolated from patients with acute Chagas disease that had been acquired by oral transmission in the state of Santa Catarina, Brazil (2005) and two isolates that had been obtained from a marsupial (Didelphis aurita) and a vector (Triatoma tibiamaculata). These strains were characterised through their biological behaviour and isoenzymic profiles and genotyped according to the new Taxonomy Consensus (2009) based on the discrete typing unities, that is, T. cruzi genotypes I-VI. All strains exhibited the biological behaviour of biodeme type II. In six isolates, late peaks of parasitaemia, beyond the 20th day, suggested a double infection with biodemes II + III. Isoenzymes revealed Z2 or mixed Z1 and Z2 profiles. Genotyping was performed using three polymorphic genes (cytochrome oxidase II, spliced leader intergenic region and 24Sα rRNA) and the restriction fragment length polymorphism of the kDNA minicircles. Based on these markers, all but four isolates were characterised as T. cruzi II genotypes. Four mixed populations were identified: SC90, SC93 and SC97 (T. cruzi I + T. cruzi II) and SC95 (T. cruzi I + T. cruzi VI). Comparison of the results obtained by different methods was essential for the correct identification of the mixed populations and major lineages involved indicating that characterisation by different methods can provide new insights into the relationship between phenotypic and genotypic aspects of parasite behaviour.
Keywords: Trypanosoma cruzi strains
Oral transmission
Biodemes
Genotyping
Taxonomy Consensus 2009
DeCS: Doença de Chagas/parasitologia
Trypanosoma cruzi /genética
Animais
Brasil /epidemiologia
Doença de Chagas /epidemiologia
Doença de Chagas /transmissão
Consenso
DNA de Protozoário /genética
Didelphis /parasitologia
Surtos de Doenças
Reservatórios de Doenças /parasitologia
Genótipo
Humanos
Insetos Vetores /parasitologia
RNA Ribossômico /genética
Triatoma /parasitologia
Trypanosoma cruzi /classificação
Trypanosoma cruzi /patogenicidade
Issue Date: 2011
Citation: ANDRADE, S.G. et al. Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009). Memórias do Instituto Oswaldo Cruz, v. 106, n. 8, p. 948-56, dec. 2011.
ISSN: 1678-8060
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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