Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5856
Title: An Evaluation of the Spontaneous Proliferation of Peripheral Blood Mononuclear Cells in HTLV-1-Infected Individuals Using Flow Cytometry
Authors: Pinto, Lorena Ana
Castro Filho, Bernardo Galvão
Soares, Milena Botelho Pereira
Grassi, Maria Fernanda Rios
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Abstract: The spontaneous proliferation of peripheral blood mononuclear cells (PBMCs) is a hallmark of the human T-lymphotropic virus (HTLV) type-1. Cell proliferation is usually measured using a [(3)H]thymidine incorporation assay. This study aims to quantify the spontaneous proliferation of PBMCs using flow cytometry. PBMCs were cultured for 24 to 120 hours in the presence of 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). For comparison, PBMCs were also cultured with [(3)H]thymidine. The cutoff values for spontaneous proliferation were >0.06 for the division index and >5.8% for the percentage of divided cells. Sixty-two percent of HTLV-1-infected individuals presented spontaneous proliferation of PBMCs, which was detected in the first 24 hours. Moreover, proliferation was detected in CD4(+) and CD8(+) T-lymphocyte subsets. A positive correlation was found between the division index and [(3)H]thymidine incorporation. This method may prove useful to better understand the phenomenon of spontaneous proliferation of PBMC of patients infected with HTLV-1.
Keywords: HTLV-1
Cell proliferation
Flow Cytometry
Mononuclear Cells
keywords: HTLV-1
Proliferação celular
Citometria de fluxo
Células mononucleares
Issue Date: 2011
Citation: PINTO, L. A. et al. An Evaluation of the spontaneous proliferation of peripheral blood mononuclear cells in HTLV-1-infected individuals Using Flow Cytometry. ISRN Oncology, v. 2011, p. 1-6, 2011.
DOI: 10.5402/2011/326719
ISSN: 2090-567X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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