Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5862
Title: Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
Authors: Tavares, Natalia Machado
Silva, Robson Amaro Augusto da
Costa, Dirceu Joaquim
Pitombo, Maiana A
Fukutani, Kiyoshi Ferreira
Miranda, José Carlos
Valenzuela, Jesus G
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral Netto, Manoel
Brodskyn, Claudia Ida
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Campus Anisio Teixeira. Vitoria da Conquista, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
National Institute of Allergy and Infectious Disease. National Institutes of Health. Vector Molecular Biology Unit. Rockville, MD, United States of America
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Leishmania transmission occurs in the presence of insect saliva. Immunity to Phlebotomus papatasi or Lutzomyia longipalpis saliva or salivary components confers protection against an infection by Leishmania in the presence of the homologous saliva. However, immunization with Lutzomyia intermedia saliva did not protect mice against Leishmania braziliensis plus Lu. intermedia saliva. In the present study, we have studied whether the immunization with Lu. longipalpis saliva or a DNA plasmid coding for LJM19 salivary protein would be protective against L. braziliensis infection in the presence of Lu. intermedia saliva, the natural vector for L. braziliensis. METHODOLOGY/PRINCIPAL FINDINGS: Immunization with Lu. longipalpis saliva or with LJM19 DNA plasmid induced a Delayed-Type Hypersensitivity (DTH) response against Lu. longipalpis as well as against a Lu. intermedia saliva challenge. Immunized and unimmunized control hamsters were then intradermally infected in the ears with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia saliva. Animals immunized with Lu. longipalpis saliva exhibited smaller lesion sizes as well as reduced disease burdens both at lesion site and in the draining lymph nodes. These alterations were associated with a significant decrease in the expression levels of IL-10 and TGF-ß. Animals immunized with LJM19 DNA plasmid presented similar findings in protection and immune response and additionally increased IFN-γ expression. CONCLUSIONS/SIGNIFICANCE: Immunization with Lu. longipalpis saliva or with a DNA plasmid coding LJM19 salivary protein induced protection in hamsters challenged with L. braziliensis plus Lu. intermedia saliva. These findings point out an important role of immune response against saliva components, suggesting the possibility to develop a vaccine using a single component of Lu. longipalpis saliva to generate protection against different species of Leishmania, even those transmitted by a different vector.
DeCS: Transmissão de Doença Infecciosa/prevenção & controle
Leishmania braziliensis/imunologia
Leishmaniose Cutânea/prevenção & controle
Vacinas Protozoárias/imunologia
Psychodidae/química
Proteínas e Peptídeos Salivares/imunologia
Vacinas de DNA/imunologia
Animais
Cricetinae
Feminino
Interferon gama/secreção
Interleucina-10/secreção
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/patologia
Leishmaniose Cutânea/transmissão
Linfonodos/patologia
Masculino
Mesocricetus
Vacinas Protozoárias/administração & dosagem
Saliva/química
Saliva/imunologia
Proteínas e Peptídeos Salivares/isolamento & purificação
Pele/parasitologia
Pele/patologia
Vacinas de DNA/administração & dosagem
Issue Date: 2011
Citation: TAVARES, N.M. et al. Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia. PLoS Neglected Tropical Diseases, v. 5, n. 5, p. e1169, 2011.
DOI: 10.1371/journal.pntd.0001169
ISSN: 1935-2735
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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