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https://www.arca.fiocruz.br/handle/icict/58740
INTERACTION OF BIOMPHALARIA CELLS IN PRIMARY CULTURES WITH SCHISTOSOMA MANSONI SPOROCYSTS
Biomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
Author
Affilliation
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil
Abstract
Introduction: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. Methods: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. tenagophila HM and B. glabrata BH) strains of Biomphalaria were exposed to S. mansoni sporocysts. Results: S. mansoni sporocysts were surrounded and encapsulated exclusively by cells derived from the digestive gland (DG) of B. tenagophila Taim. The process was followed by a marked decrease in the number of free sporocysts in the culture medium. The morphological characteristics of DG-derived cells in culture have been described. Conclusions: Cells derived from DG (but not SK) primary cultures of B. tenagophila Taim may participate in S. mansoni sporocyst control.
Keywords
Biomphalaria tenagophilaBiomphalaria glabrata
Schistosoma mansoni
Digestive gland
Saccular kidney
Primary cell culture
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