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2099-12-31
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SEQUENCE AND STRUCTURAL ANALYSIS OF THE 5 ' NONCODING REGION OF HEPATITIS C VIRUS IN PATIENTS WITH CHRONIC INFECTION
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Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil
Universidade de Sao Paulo. Instituto de Matematica e Estatıstica. Sao Paulo, SP, Brazil/Universidade de Sao Paulo. Instituto de Psiquiatria. Sao Paulo, SP, Brazil
Universidade de Sao Paulo. Instituto de Matematica e Estatıstica. Sao Paulo, SP, Brazil/Universidade de Sao Paulo. Instituto de Psiquiatria. Sao Paulo, SP, Brazil
Universidade Federal de Minas Gerais. Instituto Alfa de Gastroenterologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil
Universidade de Sao Paulo. Instituto de Matematica e Estatıstica. Sao Paulo, SP, Brazil/Universidade de Sao Paulo. Instituto de Psiquiatria. Sao Paulo, SP, Brazil
Universidade de Sao Paulo. Instituto de Matematica e Estatıstica. Sao Paulo, SP, Brazil/Universidade de Sao Paulo. Instituto de Psiquiatria. Sao Paulo, SP, Brazil
Universidade Federal de Minas Gerais. Instituto Alfa de Gastroenterologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil
Abstract
Hepatitis C virus (HCV), exhibits considerable genetic diversity, but presents a relatively well conserved 5 ' noncoding region (5 ' NCR) among all genotypes. In this study, the structural features and translational efficiency of the HCV 5 ' NCR sequences were analyzed using the programs RNAfold, RNAshapes and RNApdist and with a bicistronic dual luciferase expression system, respectively. RNA structure prediction software indicated that base substitutions will alter potentially the 5 ' NCR structure. The heterogeneous sequence observed on 5 ' NCR led to important changes in their translation efficiency in different cell culture lines. Interactions of the viral RNA with cellular transacting factors may vary according to the cell type and viral genome polymorphisms that may result in the translational efficiency observed.
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