Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5950
Title: HEV, TTV and GBV-C/HGV markers in patients with acute viral hepatitis
Authors: Lyra, André de Castro
Pinho, J. R. R.
Silva, Luciano Kalabric
Sousa, L.
Saraceni, C. P.
Braga, Eduardo Lorens
Pereira, J. E.
Zarife, Maria Alice Sant’Anna
Reis, Mitermayer Galvão dos
Lyra, Luiz Guilherme Costa
Silva, L. C. da
Carrilho, Flair José
Affilliation: Universidade de São Paulo. Departamento de Gastroenterologia. São Paulo, SP, Brasil.
Instituto Adolfo Lutz. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Instituto Adolfo Lutz. São Paulo, SP, Brasil.
Instituto Adolfo Lutz. São Paulo, SP, Brasil.
Universidade Federal da Bahia. Serviço de Gastro-Hepatologia. Salvador, BA, Brasil.
Universidade Federal da Bahia. Serviço de Gastro-Hepatologia. Salvador, Bahia, Brasil
Laboratório Central da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Serviço de Gastro-Hepatologia. Salvador, BA, Brasil.
Universidade de São Paulo. Departamento de Gastroenterologia. São Paulo, SP, Brasil.
Universidade de São Paulo. Departamento de Gastroenterologia. São Paulo, SP, Brasil.
Abstract: The aim of the present study was to evaluate the prevalence of HEV, TTV and GBV-C/GBV-C/HGV in patients with acute viral hepatitis A, B and non-A-C. We evaluated sera of 94 patients from a sentinel program who had acute hepatitis A (N = 40), B (N = 42) and non-A-C (N = 12); 71 blood donors served as controls. IgM and anti-HEV IgG antibodies were detected by enzyme immunoassay using commercial kits. TTV and GBV-C/HGV were detected by nested PCR; genotyping was done by sequencing and phylogenetic analysis. Anti-HEV IgG was present in 38, 10 and 17 percent of patients with hepatitis A, B and non-A-C. Four patients with hepatitis A and 1 with non-A-C hepatitis also had anti-HEV IgM detected in serum. TTV was detected in 21 percent of patients with acute hepatitis and in 31 percent of donors. GBV-C/HGV was detected in 9 percent of patients with hepatitis, and in 10 percent of donors. We found TTV isolates of genotypes 1, 2, 3, and 4 and GBV-C/HGV isolates of genotypes 1 and 2. Mean aminotransferase levels were lower in patients who were TTV or GBV-C/HGV positive. In conclusion, the detection of anti-HEV IgM in some acute hepatitis A cases suggests co-infection with HEV and hepatitis E could be the etiology of a few cases of sporadic non-A-C hepatitis in Salvador, Brazil. TTV genotype 1, 2, 3 and 4 isolates and GBV-C/HGV genotype 1 and 2 strains are frequent in the studied population. TTV and GBV-C/HGV infection does not appear to have a role in the etiology of acute hepatitis.
Keywords: Acute hepatitis
Hepatitis E
TTV
GBV-C/HGV
Genotype
Brazil
DeCS: Hepatite Viral Humana/virologia
Anticorpos Anti-Hepatite/sangue
Vírus da Hepatite E/imunologia
Torque teno virus/imunologia
Vírus GB C/imunologia
Hepatite Viral Humana/diagnóstico
Hepatite Viral Humana/epidemiologia
Vírus da Hepatite E/genética
Torque teno virus/genética
Vírus GB C/genética
Marcadores Biológicos
Vigilância de Evento Sentinela
Estudos de Casos e Controles
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Reação em Cadeia da Polimerase
Genótipo
Índice de Gravidade de Doença
Doença Aguda
Prevalência
Brasil/epidemiologia
Humanos
Masculino
Feminino
Issue Date: 2005
Citation: LYRA, A. C. et al. HEV, TTV and GBV-C/HGV markers in patients with acute viral hepatitis. Brazilian Journal of Medical Biological Research, v. 38, n. 5 p. 767-775, May 2005.
ISSN: 0100-879X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Lyra, AC et al HEV, TTV and GBV.pdf599.82 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.