In this paper, pyrrole compounds were synthesized based on the Knorr method consisting of a cyclocondensation reaction between α-amino-β-ketoester with β-ketoesters and β-diketone. Different pyrrole derivatives were obtained by varying the composition of the ester group into β-ketoesters through transesterification reactions. Furthermore, after the synthesis of pyrrole nuclei, acid hydrolysis reactions, decarboxylation of the tert-butyl group, electrophilic aromatic substitution using N-bromosuccinimide or reaction with thiosemicarbazide were explored to vary the composition of the α group in relation to the nitrogen heteroatom of the pyrrole nuclei. Regarding the anti-Trypanosoma cruzi activity of pyrrole derivatives, 4 compounds synthesized were active, with IC50 ranging between 14.1 and >50 µM.