| Author | Oliveira, Milton A. P. | |
| Author | Santiago, Helton da Costa | |
| Author | Lisboa, Cristiane Rabelo | |
| Author | Cerávolo, Isabela Pena | |
| Author | Trinchieri, Giorgio | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Vieira, Leda Quercia | |
| Access date | 2023-09-20T17:38:27Z | |
| Available date | 2023-09-20T17:38:27Z | |
| Document date | 2000 | |
| Citation | OLIVEIRA, Milton A. P. et al. Leishmania sp: comparative study with Toxoplasma gondii and Trypanosoma cruzi in their ability to initialize IL-12 and IFN-gamma synthesis. Exp Parasitol., v. 95, n. 2, p. 96-105, 2000. doi: 10.1006/expr.2000.4523. | en_US |
| ISSN | 0014-4894 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60511 | |
| Language | eng | en_US |
| Publisher | Academic Press | en_US |
| Rights | restricted access | |
| Title | Leishmania sp: comparative study with Toxoplasma gondii and Trypanosoma cruzi in their ability to initialize IL-12 and IFN-gamma synthesis. | en_US |
| Type | Article | |
| Abstract | We compared in vitro and in vivo induction of IL-12 (p40) and IFN-gamma by mouse cells stimulated with Toxoplasma gondii, Trypanosoma cruzi, and different species of Leishmania. Spleen cells cultured in vitro with T. cruzi or T. gondii, but not with Leishmania, produced IL-12 (p40) and IFN-gamma. Accordingly, IL-12 (p40) was produced by macrophages stimulated in vitro with live T. cruzi or T. gondii or membrane glycoconjugates obtained from trypomastigotes or tachyzoites. No IL-12 production was detected when macrophages were stimulated with live parasites or glycoconjugates from Leishmania, regardless of priming with IFN-gamma. In vivo, only T. cruzi and T. gondii induced the synthesis of IL-12 and IFN-gamma by mouse spleen cells after intraperitoneal injection of parasites. When injected subcutaneously, live Leishmania sp. induced IL-12 (p40) and IFN-gamma production by draining lymph node cells, albeit the levels were slightly lower than those induced by infection with T. gondii or T. cruzi using the same route. Together our results indicate that under different conditions, the intracellular protozoa T. gondii and T. cruzi are more potent stimulators of IL-12 and IFN-gamma synthesis by host immune cells than parasites of the genus Leishmania. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Wistar Institute of Anatomy and Biology. Philadelphia, Pennsylvania, U.S.A | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departmento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departmento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | en_US |
| Subject | Trypanosoma cruzi | en_US |
| Subject | Leishmania amazonensis | en_US |
| Subject | Leishmania major | en_US |
| Subject | Toxoplasma gondii | en_US |
| Subject | Th1 | en_US |
| Subject | macrophage | en_US |
| Subject | membrane glycoconjugates | en_US |
| Subject | cell-mediated immunity (CMI) | en_US |
| Subject | interleukin-4 (IL-4) | en_US |
| Subject | interleukin-12 (IL-12) | en_US |
| Subject | interferon-γ (IFN-γ) | en_US |
| Subject | soluble antigen of T. gondii (STAg) | en_US |
| Subject | concanavalin A (Con A) | en_US |
| Embargo date | 2099-12-31 | |
