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2099-12-31
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LEISHMANIA SP: COMPARATIVE STUDY WITH TOXOPLASMA GONDII AND TRYPANOSOMA CRUZI IN THEIR ABILITY TO INITIALIZE IL-12 AND IFN-GAMMA SYNTHESIS.
Leishmania amazonensis
Leishmania major
Toxoplasma gondii
Th1
macrophage
membrane glycoconjugates
cell-mediated immunity (CMI)
interleukin-4 (IL-4)
interleukin-12 (IL-12)
interferon-γ (IFN-γ)
soluble antigen of T. gondii (STAg)
concanavalin A (Con A)
Author
Affilliation
Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil
Wistar Institute of Anatomy and Biology. Philadelphia, Pennsylvania, U.S.A
Universidade Federal de Minas Gerais. Departmento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departmento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil
Wistar Institute of Anatomy and Biology. Philadelphia, Pennsylvania, U.S.A
Universidade Federal de Minas Gerais. Departmento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departmento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Abstract
We compared in vitro and in vivo induction of IL-12 (p40) and IFN-gamma by mouse cells stimulated with Toxoplasma gondii, Trypanosoma cruzi, and different species of Leishmania. Spleen cells cultured in vitro with T. cruzi or T. gondii, but not with Leishmania, produced IL-12 (p40) and IFN-gamma. Accordingly, IL-12 (p40) was produced by macrophages stimulated in vitro with live T. cruzi or T. gondii or membrane glycoconjugates obtained from trypomastigotes or tachyzoites. No IL-12 production was detected when macrophages were stimulated with live parasites or glycoconjugates from Leishmania, regardless of priming with IFN-gamma. In vivo, only T. cruzi and T. gondii induced the synthesis of IL-12 and IFN-gamma by mouse spleen cells after intraperitoneal injection of parasites. When injected subcutaneously, live Leishmania sp. induced IL-12 (p40) and IFN-gamma production by draining lymph node cells, albeit the levels were slightly lower than those induced by infection with T. gondii or T. cruzi using the same route. Together our results indicate that under different conditions, the intracellular protozoa T. gondii and T. cruzi are more potent stimulators of IL-12 and IFN-gamma synthesis by host immune cells than parasites of the genus Leishmania.
Keywords
Trypanosoma cruziLeishmania amazonensis
Leishmania major
Toxoplasma gondii
Th1
macrophage
membrane glycoconjugates
cell-mediated immunity (CMI)
interleukin-4 (IL-4)
interleukin-12 (IL-12)
interferon-γ (IFN-γ)
soluble antigen of T. gondii (STAg)
concanavalin A (Con A)
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