| Author | Sucupira, Pedro Henrique Ferreira | |
| Author | Ferreira, Milene Silveira | |
| Author | Coutinho-da-Silva, Mikelly Santos | |
| Author | Bicalho, Kelly Alves | |
| Author | Campi-Azevedo, Ana Carolina | |
| Author | Brito-de-Sousa, Joaquim Pedro | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Rios, Maria | |
| Author | Konduru, Krishnamurthy | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Reis, Jordana Grazziela Alves Coelho dos | |
| Author | Antonelli, Lis Ribeiro do Valle | |
| Author | Rezende, Vitor Bortolo de | |
| Author | Melo, Fernanda Ludolf Ribeiro de | |
| Author | Garcia, Cristiana Couto | |
| Author | Silva-Andrade, Jesuanne Carla | |
| Author | Rocha, Ismael Artur da Costa | |
| Author | Rocha, Lucia Alves da | |
| Author | Silva, Valderjane Aprigio | |
| Author | Pinto, Sérgio Damasceno | |
| Author | Melo, Sabrina Araújo de | |
| Author | Costa, Allyson Guimarães | |
| Author | Gomes, Matheus de Souza | |
| Author | Amaral, Laurence Rodrigues | |
| Author | Bertarini, Pedro Luiz Lima | |
| Author | Furtado, Erilene Cristina da Silva | |
| Author | Silva, Eliana Vieira Pinto da | |
| Author | Ramos, Bruna Alves | |
| Author | Santos, Éder Barros Dos | |
| Author | Freitas, Maria Nazaré Oliveira | |
| Author | Faria, Ana Maria Caetano | |
| Author | Vasconcelos, Pedro Fernando da Costa | |
| Author | Bastos, Michele de Souza | |
| Author | Martins, Livia Carício | |
| Author | Martins Filho, Olindo Assis | |
| Author | Araújo, Márcio Sobreira Silva | |
| Access date | 2023-10-04T12:31:45Z | |
| Available date | 2023-10-04T12:31:45Z | |
| Document date | 2023 | |
| Citation | SUCUPIRA, Pedro Henrique Ferreira et al. Serotype-associated immune response and network immunoclusters in children and adults during acute Dengue virus infection. Cytokine, v. 169, p. 156306, 2023. https://doi.org/10.1016/j.cyto.2023.156306 | en_US |
| ISSN | 1043-4666 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/60680 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| Title | Serotype-associated immune response and network immunoclusters in children and adults during acute Dengue virus infection | en_US |
| Type | Article | |
| Abstract | The present study was designed as an exploratory investigation to characterize the overall profile of chemokines, growth factors, and pro-inflammatory/regulatory cytokines during acute DENV infection according to DENV-1, DENV-2, DENV-4 serotypes and age: children: <1-10-year-old (yo); adolescents:11-20 yo; adults 21-40 yo; and older adults: 41-75 yo. The levels of soluble immunemediators were measured in serum by high-throughput microbeads array in 636 subjects including 317 DENV-infected and 319 age-matching non-infected control (NI). Overall, most soluble mediators were increased in DENV-infected patients as compared to NI group regardless of age and DENV serotype, with high magnitude order of increase for CCL2, CXCL10, IL-1β, IFN-γ, IL1-Ra (fold change >3x), except PDGF in which no fold change was observed. Moreover, despite the age ranges, DENV-1 and DENV-4 presented increased levels of VEGF, IL-6, and TNF-α in serum but decreased levels of PDGF, while DENV-2 exhibited increased levels of CXCL8, CCL4, and IL-12. Noteworthy was that DENV-2 showed increased levels of IL-12, IL-15, IL-17, IL-4, IL-9, and IL-13, and maintained an unaltered levels of PDGF at younger ages (<1-10 yo and 11-20 yo), whereas in older ages (21-40 yo and 41-75 yo), the results showed increased levels of CCL2, IL-6, and TNF-α, but lower levels of PDGF. In general, DENV infection at younger age groups exhibited more complex network immunoclusters as compared to older age groups. Multivariate analysis revealed a clustering of DENV cases according to age for a set of soluble mediators especially in subjects infected with DENV-2 serotype. Altogether, our findings demonstrate that the profile of circulating soluble mediators differs substantially in acute DENV according to age and DENV serotypes suggesting the participation of serotype-associated immune response, which may represent a potential target for development of therapeutics and could be used to assist medical directive for precise clinical management of severe cases. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Office of Blood Research and Review. Center for Biologics Evaluation and Research. U.S. Food and Drug Administration. Silver Spring, MD, USA. | en_US |
| Affilliation | Office of Blood Research and Review. Center for Biologics Evaluation and Research. U.S. Food and Drug Administration. Silver Spring, MD, USA. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade Federal do Amazonas. Manaus, AM, Brazil | en_US |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil. | en_US |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brazil. | en_US |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade Federal do Amazonas. Manaus, AM, Brazil/Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brazil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brazil/Universidade Federal de Uberlândia. Faculdade de Engenharia Elétrica. Patos de Minas. Laboratório de Tecnologias Urbanas e Rurais. MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brazil/Universidade Federal de Uberlândia. Faculdade de Engenharia Elétrica. Patos de Minas. Laboratório de Tecnologias Urbanas e Rurais. MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brazil/Universidade Federal de Uberlândia. Faculdade de Engenharia Elétrica. Patos de Minas. Laboratório de Tecnologias Urbanas e Rurais. MG, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil/Universidade do Estado do Pará. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Belém, PA, Brazil. | en_US |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade Federal do Amazonas. Manaus, AM, Brazil. | en_US |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Age | en_US |
| Subject | Chemokines | en_US |
| Subject | Cytokines | en_US |
| Subject | DENV Serotypes | en_US |
| Subject | Dengue infection | en_US |
| Embargo date | 3100-12-31 | |
