Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/61509
Type
ArticleCopyright
Open access
Collections
- INI - Artigos de Periódicos [3646]
- IOC - Artigos de Periódicos [12977]
- PE - IAM - Artigos de Periódicos [1186]
Metadata
Show full item record10
CITATIONS
10
Total citations
10
Recent citations
n/a
Field Citation Ratio
1.1
Relative Citation Ratio
ZIKA-SPECIFIC NEUTRALIZING ANTIBODIES TARGETING INTER-DIMER ENVELOPE EPITOPE
X-ray crystallography
Zika virus
Dengue virus
Flavivirus
Monoclonal antibodies
Neutralizing antibodies
Rhesus macaque
Structural biology
Author
Sankhala, Rajeshwer S.
Dussupt, Vincent
Donofrio, Gina
Gromowski, Gregory D.
Barrera, Rafael A. De La
Larocca, Rafael A.
Mendez-Rivera, Letzibeth
Lee, Anna
Choe, Misook
Zaky, Weam
Mantus, Grace
Jensen, Jaime L.
Chen, Wei-Hung
Gohain, Neelakshi
Bai, Hongjun
McCracken, Michael K.
Mason, Rosemarie D.
Leggat, David
Slike, Bonnie M.
Tran, Ursula
Jian, Ningbo
Abbink, Peter
Peterson, Rebecca
Mendes, Eric Araujo
Franca, Rafael Freitas de Oliveira
Calvet, Guilherme Amaral
Filippis, Ana Maria Bispo de
McDermott, Adrian
Roederer, Mario
Hernandez, Mayda
Albertus, Amie
Davidson, Edgar
Doranz, Benjamin J.
Rolland, Morgane
Robb, Merlin L.
Lynch, Rebecca M.
Barouch, Dan H.
Jarman, Richard G.
Thomas, Stephen J.
Modjarrad, Kayvon
Michael, Nelson L.
Krebs, Shelly J.
Joyce, Gordon
Dussupt, Vincent
Donofrio, Gina
Gromowski, Gregory D.
Barrera, Rafael A. De La
Larocca, Rafael A.
Mendez-Rivera, Letzibeth
Lee, Anna
Choe, Misook
Zaky, Weam
Mantus, Grace
Jensen, Jaime L.
Chen, Wei-Hung
Gohain, Neelakshi
Bai, Hongjun
McCracken, Michael K.
Mason, Rosemarie D.
Leggat, David
Slike, Bonnie M.
Tran, Ursula
Jian, Ningbo
Abbink, Peter
Peterson, Rebecca
Mendes, Eric Araujo
Franca, Rafael Freitas de Oliveira
Calvet, Guilherme Amaral
Filippis, Ana Maria Bispo de
McDermott, Adrian
Roederer, Mario
Hernandez, Mayda
Albertus, Amie
Davidson, Edgar
Doranz, Benjamin J.
Rolland, Morgane
Robb, Merlin L.
Lynch, Rebecca M.
Barouch, Dan H.
Jarman, Richard G.
Thomas, Stephen J.
Modjarrad, Kayvon
Michael, Nelson L.
Krebs, Shelly J.
Joyce, Gordon
Affilliation
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Integral Molecular. Philadelphia, USA / Present address: Hansoh Bio. Rockville, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA.
Pilot Bioproduction Facility. Walter Reed Army Institute of Research. Silver Spring, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA / Present address: Moderna, Cambridge, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA / Prellis Biologics, Berkeley, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / Vaccine Research Center. Bethesda, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
George Washington University School of Medicine & Health Sciences. Washington, USA / Vaccine Research Center. Bethesda, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA.
Vaccine Research Center. Bethesda, USA.
Vaccine Research Center. Bethesda, USA / US Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA.
Oswaldo Cruz Foundation. Institute Aggeu Magalhaes. Recife, PE, Brazil.
Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil.
Vaccine Research Center. Bethesda, USA / Present address: Sanofi. Swiftwater, USA.
Vaccine Research Center. Bethesda, USA.
Integral Molecular. Philadelphia, USA.
Integral Molecular. Philadelphia, USA.
Integral Molecular. Philadelphia, USA.
Integral Molecular. Philadelphia, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
George Washington University School of Medicine & Health Sciences. Washington, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA / Ragon Institute of MGH, MIT, and Harvard. Cambridge, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA / SUNY, Upstate Medical University. Syracuse, NY, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Pfizer. Pearl River, NY, USA.
Center of Infectious Disease Research. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA.
Pilot Bioproduction Facility. Walter Reed Army Institute of Research. Silver Spring, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA / Present address: Moderna, Cambridge, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA / Prellis Biologics, Berkeley, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / Vaccine Research Center. Bethesda, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
George Washington University School of Medicine & Health Sciences. Washington, USA / Vaccine Research Center. Bethesda, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA.
Vaccine Research Center. Bethesda, USA.
Vaccine Research Center. Bethesda, USA / US Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA.
Oswaldo Cruz Foundation. Institute Aggeu Magalhaes. Recife, PE, Brazil.
Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil.
Vaccine Research Center. Bethesda, USA / Present address: Sanofi. Swiftwater, USA.
Vaccine Research Center. Bethesda, USA.
Integral Molecular. Philadelphia, USA.
Integral Molecular. Philadelphia, USA.
Integral Molecular. Philadelphia, USA.
Integral Molecular. Philadelphia, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA.
George Washington University School of Medicine & Health Sciences. Washington, USA.
Center for Virology and Vaccine Research. Beth Israel Deaconess Medical Center. Harvard Medical School. Boston, USA / Ragon Institute of MGH, MIT, and Harvard. Cambridge, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA.
Viral Diseases Branch. Walter Reed Army Institute of Research. Silver Spring, USA / SUNY, Upstate Medical University. Syracuse, NY, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Pfizer. Pearl River, NY, USA.
Center of Infectious Disease Research. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Emerging Infectious Disease Branch. Walter Reed Army Institute of Research. Silver Spring, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine. Bethesda, USA / U.S. Military HIV Research Program. Walter Reed Army Institute of Research. Silver Spring, USA.
Abstract
Zika virus (ZIKV) is an emerging pathogen that causes devastating congenital defects. The overlapping epidemiology and immunologic cross-reactivity between ZIKV and dengue virus (DENV) pose complex challenges to vaccine design, given the potential for antibody-dependent enhancement of disease. Therefore, classification of ZIKV-specific antibody targets is of notable value. From a ZIKV-infected rhesus macaque, we identify ZIKV-reactive B cells and isolate potent neutralizing monoclonal antibodies (mAbs) with no cross-reactivity to DENV. We group these mAbs into four distinct antigenic groups targeting ZIKV-specific cross-protomer epitopes on the envelope glycoprotein. Co-crystal structures of representative mAbs in complex with ZIKV envelope glycoprotein reveal envelope-dimer epitope and unique dimer-dimer epitope targeting. All four specificities are serologically identified in convalescent humans following ZIKV infection, and representative mAbs from all four groups protect against ZIKV replication in mice. These results provide key insights into ZIKV-specific antigenicity and have implications for ZIKV vaccine, diagnostic, and therapeutic development.
Keywords
ImmunologyX-ray crystallography
Zika virus
Dengue virus
Flavivirus
Monoclonal antibodies
Neutralizing antibodies
Rhesus macaque
Structural biology
Share