The intra-institutional transport of biological material between laboratories within the same institution is a common practice, but is often carried out inappropriately, posing risks to the safety of employees, passers-by and the environment. Unlike interinstitutional transport, this activity is not regulated, and the lack of procedures and specific cases for this purpose in the Brazilian market exacerbates the challenges. In this context, the standardization of the procedure and isothermal cases becomes essential to guarantee the safety and quality of transport. The Biosafety Advisory Department of the Fiocruz Immunobiological Technology Institute developed a Standard Operating Procedure (SOP) accompanied by a playful booklet to promote employee adherence to bioprotective measures. The SOP covers everything from safely packaging the material in primary packaging to disinfecting the isothermal case after each transport. Highlights include the use of watertight secondary packaging, impact-resistant suitcases capable of maintaining temperature, and the mandatory use of PPE during cleaning and decontamination of the materials used. The use of playful tools, such as the booklet, proves to be an effective strategy for training and awareness, increasing employees' understanding and adherence to biosafety practices. In addition to the SOP and the booklet, the distribution of these materials was extended to laboratories, reaching a greater number of employees compared to traditional training methods. The initiative seeks to ensure people's safety, preserve the environment and guarantee the integrity of biological material during intra-institutional transport.