| Author | Vance, Gillian M. | |
| Author | Khouri, Mariana I. | |
| Author | Silva Neto, Almiro Pires da | |
| Author | James, Sally | |
| Author | Leite, Luciana C. C. | |
| Author | Farias, Leonardo Paiva | |
| Author | Wilson, R. Alan | |
| Access date | 2024-05-23T14:04:56Z | |
| Available date | 2024-05-23T14:04:56Z | |
| Document date | 2024 | |
| Citation | VANCE, Gillian M. et al. Antigenic epitope targets of rhesus macaques self-curing from Schistosoma mansoni infection. Frontiers in Immunology, v. 14, p. 1-12, 2024. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64203 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo FAPESP).
Fundação Butantan.
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). | en_US |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Mapeamento de epítopos | en_US |
| Subject in Portuguese | Proteínas tegumentares | en_US |
| Subject in Portuguese | Proteínas do trato alimentar s | en_US |
| Subject in Portuguese | Alvos antigênicos | en_US |
| Subject in Portuguese | Glândulas esofágicas | en_US |
| Subject in Portuguese | Matriz de peptídeo | en_US |
| Title | Antigenic epitope targets of rhesus macaques self-curing from Schistosoma mansoni infection | en_US |
| Type | Article | en_US |
| Abstract | The self-cure of rhesus macaques from a schistosome infection and their subsequent strong immunity to a cercarial challenge should provide novel insights into the way these parasites can be eliminated by immunological attack. High-density arrays comprising overlapping 15-mer peptides from target proteins printed on glass slides can be used to screen sera from host species to determine antibody reactivity at the single epitope level. Careful selection of proteins, based on compositional studies, is crucial to encompass only those exposed on or secreted from the intra-mammalian stages and is intended to focus the analysis solely on targets mediating protection. We report the results of this approach using two pools of sera from hi- and lo-responder macaques undergoing self-cure, to screen arrays comprising tegument, esophageal gland, and gastrodermis proteins. We show that, overall, the target epitopes are the same in both groups, but the intensity of response is twice as strong in the high responders. In addition, apart from Sm25, tegument proteins elicit much weaker responses than those originating in the alimentary tract, as was apparent in IFNγR KO mice. We also highlight the most reactive epitopes in key proteins. Armed with this knowledge, we intend to use multi-epitope constructs in vaccination experiments, which seek to emulate the self-cure process in experimental animals and potentially in humans. | en_US |
| Affilliation | Department of Biology. University of York. York, United Kingdom. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil. | en_US |
| Affilliation | Department of Biology. University of York. York, United Kingdom. | en_US |
| Affilliation | Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil. | en_US |
| Affilliation | Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil. | en_US |
| Affilliation | Department of Biology. University of York. York, United Kingdom / Biomedical Research Institute. University of York. York, United Kingdom. | en_US |
| Subject | Epitope mapping | en_US |
| Subject | Tegument proteins | en_US |
| Subject | Alimentary tract proteins | en_US |
| Subject | Antigenic targets | en_US |
| Subject | Esophageal glands | en_US |
| Subject | Peptide array | en_US |
| DeCS | Mapeamento de epítopos | en_US |
| DeCS | Proteínas | en_US |
| DeCS | Mucosa esofágica | en_US |
| DeCS | Peptídeos | en_US |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
