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ANTIGENIC EPITOPE TARGETS OF RHESUS MACAQUES SELF-CURING FROM SCHISTOSOMA MANSONI INFECTION
Proteínas tegumentares
Proteínas do trato alimentar s
Alvos antigênicos
Glândulas esofágicas
Matriz de peptídeo
Tegument proteins
Alimentary tract proteins
Antigenic targets
Esophageal glands
Peptide array
Author
Affilliation
Department of Biology. University of York. York, United Kingdom.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil.
Department of Biology. University of York. York, United Kingdom.
Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.
Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.
Department of Biology. University of York. York, United Kingdom / Biomedical Research Institute. University of York. York, United Kingdom.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil.
Department of Biology. University of York. York, United Kingdom.
Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.
Instituto Butantan. Laboratório de Desenvolvimento de Vacinas. São Paulo, SP, Brasil.
Department of Biology. University of York. York, United Kingdom / Biomedical Research Institute. University of York. York, United Kingdom.
Abstract
The self-cure of rhesus macaques from a schistosome infection and their subsequent strong immunity to a cercarial challenge should provide novel insights into the way these parasites can be eliminated by immunological attack. High-density arrays comprising overlapping 15-mer peptides from target proteins printed on glass slides can be used to screen sera from host species to determine antibody reactivity at the single epitope level. Careful selection of proteins, based on compositional studies, is crucial to encompass only those exposed on or secreted from the intra-mammalian stages and is intended to focus the analysis solely on targets mediating protection. We report the results of this approach using two pools of sera from hi- and lo-responder macaques undergoing self-cure, to screen arrays comprising tegument, esophageal gland, and gastrodermis proteins. We show that, overall, the target epitopes are the same in both groups, but the intensity of response is twice as strong in the high responders. In addition, apart from Sm25, tegument proteins elicit much weaker responses than those originating in the alimentary tract, as was apparent in IFNγR KO mice. We also highlight the most reactive epitopes in key proteins. Armed with this knowledge, we intend to use multi-epitope constructs in vaccination experiments, which seek to emulate the self-cure process in experimental animals and potentially in humans.
Keywords in Portuguese
Mapeamento de epítoposProteínas tegumentares
Proteínas do trato alimentar s
Alvos antigênicos
Glândulas esofágicas
Matriz de peptídeo
Keywords
Epitope mappingTegument proteins
Alimentary tract proteins
Antigenic targets
Esophageal glands
Peptide array
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