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https://www.arca.fiocruz.br/handle/icict/64344
CHARACTERIZATION OF THE GUT BACTERIAL AND VIRAL MICROBIOTA IN LATENT AUTOIMMUNE DIABETES IN ADULTS.
Author
Affilliation
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Steno Diabetes Center Copenhagen. Gentofte, Denmark/Novo Nordisk A/S. Soeborg, Denmark
Biosystems Informatics. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark/Clinical Microbiomics A/S. Copenhagen, Denmark
Center for Clinical Research and Prevention. Bispebjerg and Frederiksberg Hospital. The Capital Region. Copenhagen, Denmark/Department of Clinical Medicine. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Department of Microbiology, Immunology & Transplantation. Rega Institute. Laboratory of Clinical & Epidemiological Virology. KU Leuven, Leuven, Belgium
Center for Clinical Research and Prevention. Bispebjerg and Frederiksberg Hospital. The Capital Region. Copenhagen, Denmark/Department of Public Health. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Steno Diabetes Center Copenhagen. Gentofte, Denmark/Novo Nordisk A/S. Soeborg, Denmark
Department of Microbiology, Immunology & Transplantation. Rega Institute. Laboratory of Clinical & Epidemiological Virology. KU Leuven, Leuven, Belgium
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark/Center for Clinical Metabolic Research. Department of Medicine. Gentofte University Hospital. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark/Department of Medicine. Bornholms Hospital. Rønne, Denmark/Steno Diabetes Center Copenhagen. Gentofte, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Steno Diabetes Center Copenhagen. Gentofte, Denmark/Novo Nordisk A/S. Soeborg, Denmark
Biosystems Informatics. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark/Clinical Microbiomics A/S. Copenhagen, Denmark
Center for Clinical Research and Prevention. Bispebjerg and Frederiksberg Hospital. The Capital Region. Copenhagen, Denmark/Department of Clinical Medicine. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Department of Microbiology, Immunology & Transplantation. Rega Institute. Laboratory of Clinical & Epidemiological Virology. KU Leuven, Leuven, Belgium
Center for Clinical Research and Prevention. Bispebjerg and Frederiksberg Hospital. The Capital Region. Copenhagen, Denmark/Department of Public Health. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Steno Diabetes Center Copenhagen. Gentofte, Denmark/Novo Nordisk A/S. Soeborg, Denmark
Department of Microbiology, Immunology & Transplantation. Rega Institute. Laboratory of Clinical & Epidemiological Virology. KU Leuven, Leuven, Belgium
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark/Center for Clinical Metabolic Research. Department of Medicine. Gentofte University Hospital. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark/Department of Medicine. Bornholms Hospital. Rønne, Denmark/Steno Diabetes Center Copenhagen. Gentofte, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
The Novo Nordisk Foundation Center for Basic Metabolic Research. Faculty of Health and Medical Sciences. University of Copenhagen. Copenhagen, Denmark
Abstract
Latent autoimmune diabetes in adults (LADA) is a heterogeneous disease characterized by autoantibodies against insulin producing pancreatic beta cells and initial lack of need for insulin treatment. The aim of the present study was to investigate if individuals with LADA have an altered gut microbiota relative to non-diabetic control subjects, individuals with type 1 diabetes (T1D), and individuals with type 2 diabetes (T2D). Bacterial community profiling was performed with primers targeting the variable region 4 of the 16S rRNA gene and sequenced. Amplicon sequence variants (ASVs) were generated with DADA2 and annotated to the SILVA database. The gut virome was sequenced, using a viral particle enrichment and metagenomics approach, assembled, and quantified to describe the composition of the viral community. Comparison of the bacterial alpha- and beta-diversity measures revealed that the gut bacteriome of individuals with LADA resembled that of individuals with T2D. Yet, specific genera were found to differ in abundance in individuals with LADA compared with T1D and T2D, indicating that LADA has unique taxonomical features. The virome composition reflected the stability of the most dominant order Caudovirales and the families Siphoviridae, Podoviridae, and Inoviridae, and the dominant family Microviridae. Further studies are needed to confirm these findings.
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