In the new world, some species of sandflies are renowned as primary vectors. Such as: Lutzomyia longipalpis to Leishmania infantum, Lutzomyia intermedia, and Lutzomyia migonei to Leishmania braziliensis, but the role of those flies as a competent vector for other protozoan is unclear. Laboratory and field findings have contributed to the hypothesis of permissiveness. Here we evaluate vector competence of Lu. longipalpis, Lu. intermedia, and Lu. migonei upon infection with different species of Leishmania. We compared infection intensity with different concentration of parasites, their location in the midgut, metacyclogenesis process, and the transmission by sandfly bite effectiveness. Lu. longipalpis was able to sustain infection of all species of Leishmania at the high parasite dose, but its permissiveness is dose-dependent. Lu. intermedia only maintain a mature infection with L. braziliensis. Lu migonei was able to sustain the infection to all parasites tested, and the highest parasite infection was with L. chagasi and L. amazonensis. The transmission by the sandfly bite showed that the Lu. longipalpis is a competent vector of L. chagasi and L. major, and Lu migonei of L. chagasi and L. braziliensis parasites. However, these studies indicate the permissibility of other parasites. The Laboratory permissibility can be dose-dependent, which may explain infection in the field. Supporting that Lu 149 longipalpis and Lu migonei play a fundamental role in the epidemiology of visceral leishmaniasis in Brazil and Argentina.