| Author | Castelli, Rafael Fernando | |
| Author | Pereira, Alana | |
| Author | Amorim, Leandro Honorato de | |
| Author | Valdez, Alessandro Fernandes | |
| Author | Oliveira, Haroldo Cesar de | |
| Author | Bazioli, Jaqueline Moraes | |
| Author | Garcia, Ane Wichine Acosta | |
| Author | Klimeck, Tabata D'Maiella Freitas | |
| Author | Reis, Flavia Coelho Garcia dos | |
| Author | Andrade, Amanda Caroline Camillo de | |
| Author | Santos, Marlon Dias Mariano dos | |
| Author | Carvalho, Paulo Costa | |
| Author | Zaragoza, Oscar | |
| Author | Staats, Charley Christian | |
| Author | Nimrichter, Leonardo | |
| Author | Fill, Taícia Pacheco | |
| Author | Rodrigues, Marcio Lourenço | |
| Access date | 2024-06-20T18:24:12Z | |
| Available date | 2024-06-20T18:24:12Z | |
| Document date | 2024 | |
| Citation | CASTELLI, Rafael Fernando et al. Extracellular vesicle formation in Cryptococcus deuterogattii impacts fungal virulence. Infection and Immunity, v. 92, n. 4, p. 1-28, 12 Mar. 2024. | en_US |
| ISSN | 0019-9567 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64588 | |
| Description | Corrected and republished from: "Extracellular vesicle formation in Cryptococcus deuterogattii impacts fungal virulence" - doi: 10.1128/iai.00037-24. Epub 2024 Mar 12. | |
| Sponsorship | Ministério da Saúde (Ministry of Health). Grant 440015/2018-9 to Marcio Lourenço Rodrigues. | |
| Sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Grants 405520/2018-2, 301304/2017-3 to Marcio Lourenço Rodrigues. | |
| Sponsorship | Fundação Oswaldo Cruz (FIOCRUZ). Grants PROEP-ICC 442186/2019-3, VPPCB-007-FIO-18, VPPIS-001-FIO18 to Marcio Lourenço Rodrigues. | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). Grant 2021/00728-0 to Taícia Pacheco Fill. | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Finance Code 001 to Flavia Coelho Garcia dos Reis. | |
| Sponsorship | Ministerio de Ciencia e Innovación (MCIN). Grant PID2020-114546RB to Oscar Zaragoza. | |
| Language | eng | en_US |
| Publisher | American Society for Microbiology | en_US |
| Rights | restricted access | |
| Title | Extracellular vesicle formation in Cryptococcus deuterogattii impacts fungal virulence | en_US |
| Type | Article | |
| DOI | 10.1128/iai.00037-24 | |
| Abstract | Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii. In this study, during the phenotypic characterization of C. deuterogattii mutants expected to lack NOP16 expression, we observed a reduced EV production. Whole-genome sequencing, RNA-Seq, and cellular proteomics revealed that, contrary to our initial findings, these mutants expressed Nop16 but exhibited altered expression of 14 genes potentially involved in sugar transport. Based on this observation, we designated these mutant strains as Past1 and Past2, representing potentially altered sugar transport. Analysis of the small molecule composition of EVs produced by wild-type cells and the Past1 and Past2 mutant strains revealed not only a reduced number of EVs but also an altered small molecule composition. In a Galleria mellonella model of infection, the Past1 and Past2 mutant strains were hypovirulent. The hypovirulent phenotype was reverted when EVs produced by wild-type cells, but not mutant EVs, were co-injected with the mutant cells in G. mellonella. These results connect EV biogenesis, cargo, and cryptococcal virulence. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Parasitária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Estadual de Campinas. Instituto de Química. Campinas, SP, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Estadual de Campinas. Instituto de Química. Campinas, SP, Brasil / Universidade Estadual de Campinas. Faculdade de Ciências Farmacêuticas. Campinas, SP, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Investigaciones Biológicas Clemente Estable. Montevideo, Uruguay / Institut Pasteur de Montevideo. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Instituto de Salud Carlos III. National Centre for Microbiology. Mycology Reference Laboratory. Madrid, Spain / Instituto de Salud Carlos III. Center for Biomedical Research in Network in Infectious Diseases. Madrid, Spain. | en_US |
| Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Porto Alegre, RS, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Estadual de Campinas. Instituto de Química. Campinas, SP, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Cryptococcus | en_US |
| Subject | Extracellular vesicles | en_US |
| e-ISSN | 1098-5522 | |
| Embargo date | 2025-08-31 | |
