Schinus terebinthifolius is a medicinal plant with biological in vitro activity against clinically relevantparasites such as Leishmania amazonensis and L. chagasi. Considering its antiparasitic activity, this workaimed to investigate the in vitro effects of S. terebinthifolius fractions against P. falciparum using SYBRfluorescence tests, its cytotoxicity towards monkey kidney cells and efficacy against malaria in a murinemodel of P. berghei infection. For the first time, screening results with several extracts and fractions arereported herein. Five out of 16 fractions obtained from leaves, flowers, unripe and ripe berries withdichloromethane, ethyl acetate and butanol solvents used in plant extraction were active against P.falciparum. Two ethyl acetate fractions with no cytotoxicity in vitro presenting selectivity indexes of ≥111and ≥167 exhibited no antimalarial activity in mice at 500 mg/kg. Preliminary LCMS analysis of the ethylacetate subfractions showed antiplasmodial effect in vitro with high specific index suggesting that suchactivity is related to polyphenolics in the fractions, mainly flavonoids. Additional studies should beundertaken to identify the compound(s) with anti-malaria activity.