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Preprint
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2150-12-31
Sustainable Development Goals
06 Água potável e saneamento
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DETECTION OF MPOX VIRUS USING A WASTEWATER SURVEILLANCE APPROACH IN BRAZIL
Araujo, Juliana Calabria de et al. | Date Issued: 2024
Author
Araujo, Juliana Calabria de
Carvalho, Ana Paula A.
Leal, Cintia D.
Natividade, Manuelle
Borin, Marcus
Guerra, Augusto
Carobin, Natália
Sabino, Adriano
Almada, Mariana
Costa, Maria Cristina M.
Saia, Flavia
Frutuoso, Livia V.
Iani, Felipe Campos M.
Ribeiro, Talita E.A.
Fonseca, Vagner
Giovanetti, Marta
Alcantara, Luiz Carlos Junior
Affilliation
Department of Sanitary and Environmental Engineering. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Department of Sanitary and Environmental Engineering. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Department of Sanitary and Environmental Engineering. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Department of Social Pharmacy. College of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Department of Social Pharmacy. College of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Department of Social Pharmacy. College of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Clinical and Toxicological Analysis Department. College of Pharmacy Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Clinical and Toxicological Analysis Department. College of Pharmacy Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil
Centro Federal de Educação Tecnológica. Belo Horizonte, MG, Brazil
Centro Federal de Educação Tecnológica. Belo Horizonte, MG, Brazil
Universidade Federal de São Paulo. Baixada Santista, SP, Brazil
Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Departamento de Doenças transmissíveis. Coordenação Geral de Vigilância de Arboviroses. Brasília, DF, Brazil
Fundação Ezequiel Dias. Laboratório Central de Saúde Pública. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Laboratório Central de Saúde Pública. Belo Horizonte, MG, Brazil
Organização Pan-Americana da Saúde / Organização Mundial da Saúde. Coordenação de Vigilância, Preparação e Resposta à Emergências e Desastres. Brasilia DF, Brazil
Department of Sciences and Technologies for Sustainable Development and One Health. Universita Campus Bio-Medico di Roma. Roma, Italy
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
Abstract
Sewage surveillance can be used as an effective complementary tool for detecting pathogens in local communities, providing insights into emerging threats and aiding in the monitoring of outbreaks. In this study using qPCR and whole genomic sewage surveillance, we detected Mpox virus along with other viruses, in municipal and hospital wastewaters in Belo Horizonte, Brazil over a 9 months period (from July 2022 till March 2023). MPXV DNA detection rates varied in our study, with 19.6% (11 out of 56 samples) detected through the hybrid capture method of whole genome sequencing and 20% (12 out of 60 samples) through qPCR. In hospital wastewaters, the detection rate was higher at 40% (12 out of 30 samples), compared to 13.3%(4 out of 30 samples) in municipal wastewaters. This variation could be attributed to the relatively low number of MPXV cases reported in the city, which ranged from 106 to 341 cases during the study period, and to dilution effects, given that each of the two wastewater treatment plants (WWTP) investigated serves approximately 1.1 million inhabitants. Additionally, nine other virus families were identified in both hospitals and municipal wastewaters including Adenoviridade, Astroviridae, Caliciviridae, Picornaviridade, Polyomaviridae, Coronaviridae (which includes SARS-CoV-2), Herspesviridae, Papillomaviridae and Flaviviridae (notably including Dengue). These findings underscore the potential of genomic sewage surveillance as a robust public health tool for monitoring a wide range of viruses circulating in both community and hospitals environments including MPXV.
Keywords in Portuguese
Monkeypox virus
Keywords
Monkeypox virus
Genomic surveillance
Wastewater surveillance
Wastewater-based epidemiology
Sewage virome
 
Citation
DE ARAUJO, Juliana Calabria et al. Detection of Mpox Virus Using a Wastewater Surveillance Approach in Brazil. 2024. Available at: < https://doi.org/10.20944/preprints202405.0571.v1 >.