| Author | Morgado, Sérgio Mascarenhas | |
| Author | Fonseca, Erica Lourenço da | |
| Author | Vicente, Ana Carolina Paulo | |
| Access date | 2024-07-23T15:06:40Z | |
| Available date | 2024-07-23T15:06:40Z | |
| Document date | 2024 | |
| Citation | MORGADO, Sérgio Mascarenhas; FONSECA, Erica Lourenço da; VICENTE, Ana Carolina Paulo. Prevalence and characterization of an integrative and conjugative element carrying tet(X) gene in Elizabethkingia meningoseptica. Journal of Global Antimicrobial Resistance, p. 1-13, 14 July 2024. | |
| ISSN | 2213-7165 | |
| URI | https://www.arca.fiocruz.br/handle/icict/65096 | |
| Description | Produção científica do Laboratório de Genética Molecular de Microrganismos. | pt_BR |
| Sponsorship | This study was financed by FAPERJ - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Processo SEI-260003/019688/2022. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | open access | |
| Subject in Portuguese | Tigeciclina | pt_BR |
| Subject in Portuguese | Monooxigenase dependente de flavina | pt_BR |
| Subject in Portuguese | Surto | pt_BR |
| Subject in Portuguese | Elizabethkingia meningoseptica | pt_BR |
| Subject in Portuguese | Elemento móvel | pt_BR |
| Subject in Portuguese | Tetraciclina | pt_BR |
| Title | Prevalence and characterization of an integrative and conjugative element carrying tet(X) gene in Elizabethkingia meningoseptica | en_US |
| Type | Preprint | |
| DOI | 10.1016/j.jgar.2024.07.001 | |
| Abstract | Objectives: To investigate the tet(X) gene, a determinant of tigecycline resistance, in the emerging pathogen Elizabethkingia meningoseptica and its association with an ICE. Methods: All E. meningoseptica genomes from NCBI (n=87) were retrieved and annotated for resistome searching using the CARD database, and a phylogenic analysis was performed based on E. meningoseptica core genome. ICE was identified through comparative genomics with ICEs occurring in Elizabethkingia spp. Results: Phylogenetic analysis showed E. meningoseptica genomes from six countries distributed in different lineages, some of which persisted for years. The common resistome of these genomes included blaBlaB, blaCME, blaGOB, ranA/B, aadS, and catB (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (dfrA, ereD, blaVEB, aadS, and tet(X)). Interestingly, the tet(X) and aadS genes were located in an ICE of 49 769 bp (ICEEmSQ101), which was completely obtained from the E. meningoseptica SQ101 genome. We also raised evidence that other 27 genomes also presented this ICE. The distribution of ICEEmSQ101, carrying tet(X), was restricted to a single Chinese lineage. Conclusions: The tet(X) gene is not prevalent in the species E. meningoseptica, as previously stated for the genus Elizabethkingia since it is present basically in a single Chinese lineage. We identified that several E. meningoseptica genomes harbored an ICE that mobilized the tet(X) gene and exhibited characteristics similar to the ICEs of other Flavobacteria, which would favor their transmission in this bacteria family. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | |
| Subject | Tigecycline | en_US |
| Subject | Flavin-dependent monooxygenase | en_US |
| Subject | Outbreak | en_US |
| Subject | E. meningoseptica lineage | en_US |
| Subject | Mobile element | en_US |
| Subject | Tetracycline | en_US |
| e-ISSN | 2213-7173 | |
| Peer reviewed | Sim | |
| Publication status | Não Publicado | |
