Objectives: To investigate the tet(X) gene, a determinant of tigecycline resistance, in the emerging pathogen Elizabethkingia meningoseptica and its association with an ICE. Methods: All E. meningoseptica genomes from NCBI (n=87) were retrieved and annotated for resistome searching using the CARD database, and a phylogenic analysis was performed based on E. meningoseptica core genome. ICE was identified through comparative genomics with ICEs occurring in Elizabethkingia spp. Results: Phylogenetic analysis showed E. meningoseptica genomes from six countries distributed in different lineages, some of which persisted for years. The common resistome of these genomes included blaBlaB, blaCME, blaGOB, ranA/B, aadS, and catB (genes associated with resistance to β-lactams, aminoglycosides, and chloramphenicol). Some genomes also presented additional resistance genes (dfrA, ereD, blaVEB, aadS, and tet(X)). Interestingly, the tet(X) and aadS genes were located in an ICE of 49 769 bp (ICEEmSQ101), which was completely obtained from the E. meningoseptica SQ101 genome. We also raised evidence that other 27 genomes also presented this ICE. The distribution of ICEEmSQ101, carrying tet(X), was restricted to a single Chinese lineage. Conclusions: The tet(X) gene is not prevalent in the species E. meningoseptica, as previously stated for the genus Elizabethkingia since it is present basically in a single Chinese lineage. We identified that several E. meningoseptica genomes harbored an ICE that mobilized the tet(X) gene and exhibited characteristics similar to the ICEs of other Flavobacteria, which would favor their transmission in this bacteria family.