Sporotrichosis is caused by species of Sporothrix complex, and it affects humans and animals. Cutaneous lesions in cats carry a high parasite burden, making the cat an important source of infection. Despite the importance of cats in the zoonotic transmission ofSporothrix, little is known about the possibility of isolation of this fungus from the lesions during treatment. The purpose of this study was to evaluate the isolation of Sporothrix from ulcerated lesion in culture media during the treatment of cats with itraconazole. All cats included presented ulcerated lesions from which the isolation of the fungus in culture was conducted. All of them were treated with oral itraconazole (100 mg / cat / 24 hours). Subsequently, collection of exudate from the same lesion upon initial diagnosis was performed monthly until the outcome of the case. Cats under treatment that have presented ulcerated lesions persistent for 8 weeks or who did not attend the clinical visits were excluded. Eighty-two animals were included. In the 4th week of treatment, wound healing was observed in 44 cats and in 38 the ulcerated lesion persisted, from which in 22 fungal isolation was possible. Between the 4th and 8th week, more 14 cats had healed lesions, 6 abandoned treatment and 1 came to death; 17 presented ulcerated lesions, from which in 7 there was fungal isolation, and 11 were withdrawn from the study due to persistent lesion or changing of the therapeutic protocol. At 12th week, in 2 cats were observed the reopening of the initial lesion from where the fungus was isolated. After 8 weeks of treatment, the majority of cats showed wound healing and few had ulcerated lesions with isolation of Sporothrix, which may mean the risk reducing of transmission of the fungus between cats and humans.