| Author | Paula, Naiara Cristina Clemente dos Santos Tavares de | |
| Author | Moreira, Bernardo Pereira | |
| Author | Armstrong, Tom | |
| Author | Paiva, Clara Ênia de | |
| Author | Torres, Gabriella Parreiras | |
| Author | Oliveira, Guilherme Corrêa de | |
| Author | Gava, Sandra Grossi | |
| Author | Falconi, Franco Harald | |
| Author | Mourão, Marina Moraes | |
| Access date | 2024-09-05T12:26:06Z | |
| Available date | 2024-09-05T12:26:06Z | |
| Document date | 2019 | |
| Citation | PAULA, Naiara Cristina Clemente dos Santos Tavares de et al. SmFES and SmRAF protein kinases: roles in Schistosoma mansoni development and reproduction. In: MEDTROP: PARASITO: CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 55., 2019; CONGRESSO DA SOCIEDADE BRASILEIRA DE PARASITOLOGIA CHAGASLEISH, 26., 2019. Belo Horizonte. Anais... Belo Horizonte: SBMT, 2019. p. 106-107. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/65767 | |
| Description | 55º Congresso da Sociedade Brasileira de Medicina Tropical ◦ XXVI Congresso da Sociedade Brasileira de Parasitologia CHAGASLEISH 2019. Trabalho aparesentado no MEDTROP-Parasito 2019, realizado em Belo Horizonte no período de 28 a 31 de julho de 2019. | en_US |
| Sponsorship | Supported by: CAPES Finance Code 001 and CAPES/University of Nottingham- Drug Discovery Program 003/2014; and CNPq grant: 302518/2018-5. FIOCRUZ | en_US |
| Language | por | en_US |
| Publisher | SBMT | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Proteínas SmFES | en_US |
| Subject in Portuguese | Proteínas SmRAF | en_US |
| Subject in Portuguese | Schistosoma mansoni | en_US |
| Subject in Portuguese | MEDTROP-Parasito | en_US |
| Subject in Portuguese | Anais | en_US |
| Subject in Portuguese | Congresso | en_US |
| Title | SmFES and SmRAF protein kinases: roles in Schistosoma mansoni development and reproduction | en_US |
| Type | Papers presented at events | en_US |
| Abstract | Protein kinases (PKs) have central role in extracellular signals mediation. Accordingly, PKs are considered important to Schistosoma mansoni homeostasis maintenance and cellular adaptations since this parasite relies in specific environmental signals to induce responses through sensorial receptors and intracellular proteins. However, the role of these proteins in S. mansoni biology is not well known. This work aims at characterizing the functions of SmFES and
SmRAF protein kinases using RNA interference (RNAi) and compounds screening to identifying specific inhibitors. Thus, schistosomula and adult worms were subjected to SmFES and SmRAF knockdown by RNAi. SmFES and SmRAF
transcript levels in schistosomula and adult worms were 60%-80% reduced. SmFES knocked-down schistosomula presented significant size reduction (~10%) and 28% of mortality when compared to unspecific control group (GFP).
Mice infected with SmRAF knocked-down schistosomula showed a significant decrease in adult worms (27%) and eggs (46%) recovered from the intestine, compared to GFP. Besides, the final section from the small intestine of mice infected with SmFES and SmRAF knocked-down schistosomula presented ~25% more immature eggs than the unspecific control group and histological analyses are underway. Additionally, SmFES and SmRAF protein structures
and ATB-binding sites were predicted using Phyre2 software. In silico docking analysis were performed with 80,000 compounds from University of Nottingham MCCC Library. Compounds with predicted affinity with SmFES and SmRAF
ATP-binding sites were selected using ChemPLP Score in order to perform an in vitro viability screening in schistosomula and adult worms. These preliminary results demonstrate that SmRAF seems to play a role in S. mansoni development and reproduction during the infection of mammalian host. SmFES seems to be essential to S. mansoni reproduction, egg maturation and schistosomula survival and development in vitro. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | University Nottingham, UK. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Tecnológico Vale, Belém, PA, Brasil. | en_US |
| Affilliation | University Nottingham, UK. | en_US |
| Affilliation | University Nottingham, UK. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | en_US |
| Subject | SmRAF protein kinases | en_US |
| Subject | SmFES protein kinases | en_US |
| Subject | Schistosoma mansoni | en_US |
| Subject | Congress | en_US |
| DeCS | Proteínas Quinases | en_US |
| DeCS | Esquistossomose mansoni | en_US |
| DeCS | Congresso | en_US |
