Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/658
Title: HIV-1 infection among injection and ex-injection drug users from Rio de Janeiro, Brazil: prevalence, estimated incidence and genetic diversity
Authors: Teixeira, Sylvia Lopes Maia
Bastos, Francisco Inácio Pinkusfeld Monteiro
Dias, Paulo Roberto Telles Pires
Hacker, Mariana de Andrea Vilas-Boas
Brigido, Luis F.
Oliveira, Carmem Aparecida de F.
Bongertz, Vera
Morgado, Mariza Gonçalves
Affilliation: Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Department of Immunology. Laboratory of AIDS and Molecular Immunology. Rio de Janeiro, RJ, Brasil.
Department of Health Information, Center for Information on Science and Technology, FIOCRUZ, Rio de Janeiro, Brazil
State University of Rio de Janeiro. Rio de Janeiro Harm Reduction Project. Rio de Janeiro, RJ, Brazil.
Department of Health Information, Center for Information on Science and Technology, FIOCRUZ, Rio de Janeiro, Brazil
Government of Sao Paulo. State Secretariat of Health. Adolfo Lutz Institute. Medical Biology Division. São Paulo, SP, Brazil.
Government of Sao Paulo. State Secretariat of Health. Adolfo Lutz Institute. Medical Biology Division. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Department of Immunology. Laboratory of AIDS and Molecular Immunology. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Department of Immunology. Laboratory of AIDS and Molecular Immunology. Rio de Janeiro, RJ, Brasil.
Abstract: Background and objectives: Due to their behavioral conditions and vulnerability, injection drug users (IDUs) are prone to multiple simultaneous or sequential infections with distinct HIV-1 subtypes and variants, making them a key population for molecular epidemiology surveillance. In the present study, we evaluated HIV-1 infection seroprevalence, genetic diversity and estimated incidence among IDUs and ex-injection drug users (ex-IDUs) from Rio de Janeiro, Brazil. Study design: Six hundred and eight IDUs and ex-IDUs, recruited between 1999 and 2001, were interviewed and agreed to donate 30 ml of blood. The serologic status for HIV infection was determined by two ELISAs and confirmed by IFA. CD4+ T-cell percentages were assessed by flow cytometry. HIV-1 positive samples were submitted to viral load quantification. DNA samples were PCR amplified and HIV-1 subtypes were determined using env and gag HMA. Results and conclusions: Forty-eight (7.89%) individuals were seropositive for HIV-1 infection. The seroincidence of HIV-1 infection was estimated as 0.76%. HIV-1 env and gag subtyping identified 29 (69%) samples as belonging to subtype B, 7 (16.7%) to subtype F, and 6 (14.3%) discordant env/gag genomes infections, indicating the circulation of recombinant viruses in this population.
Keywords: HIV-1
IDU
Prevalence
Incidence
env and gag subtypes
Recombination
Issue Date: 2004
Publisher: Elsevier B.V.
Citation: TEIXEIRA, S. L. M. et al. HIV-1 infection among injection and ex-injection drug users from Rio de Janeiro, Brazil: prevalence, estimated incidence and genetic diversity. Journal of Clinical Virology, Amsterdam, v. 31, p. 221–226, 2004.
Copyright: open access
Appears in Collections:ICICT - Artigos de Periódicos

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