International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database: the quality controlled standard tool for routine identification of human and animal pathogenic fungi

Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are imeconsuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its widespread use is still limited by a lack of qualitycontrolled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on “DNA barcoding of human and animal pathogenic fungi”. A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org/ and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intraand interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular methods/genetic markers are required for their reliable identification from clinical and veterinary specimens.

Keywords in Portuguese

Identificação fúngica
Código de barras de DNA
ITS região
Banco de dados ITS de referência
Diversidade genética intraespécie/interespécie

Keywords

Fungal identification
DNA barcoding
ITS region
Reference ITS database
Intraspecies/interspecies genetic diversity

Publisher

Oxford

Later version

https://www.arca.fiocruz.br/handle/icict/32247

Citation

IRINYI, Laszlo et al. International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database: the quality controlled standard tool for routine identification of human and animal pathogenic fungi. Medical Mycology, p. 1-83, Oct. 2014.

DOI

10.1093/mmy/myv008

ISSN

1156-5233

Notes

Mauro de Medeiros Muniz e Rosely Maria Zancope-Oliveira - Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Sydney Medical SchoolWestmead Hospital, Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Westmead Millennium Institute, Sydney, Australia, 2Institut Pasteur, National Reference Center for Invasive Mycoses & Antifungals, Molecular Mycology Unit; CNRS URA3012, Paris, France, 3Mycology Research Laboratory, Department of Microbiology, Medical School, the University of Athens Hellenic Collection of Pathogenic Fungi (UOA/HCPF), National and Kapodistrian University of Athens, Athens, Greece, 4CBSKNAW, Fungal Biodiversity Centre, Utrecht, The Netherlands, 5Department of Pharmaceutical Sciences Università degli Studi di Perugia, Perugia, Italy, 6Mycology Laboratory, Department of Microbiology and Infectious Diseases, PathWest Laboratory Medicine WA, QEII Medical Centre, Nedlands, Western Australia, Australia, 7Parasitology Mycology, APHM, CHU TimoneAdultes, Marseille, France; Aix Marseille University, UMR MD3 IPTPT, Marseille, France, 8Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain, 9BCCM/IHEM, Biomedical fungi and yeasts collection, Scientific Institute of Public Health, Brussels, Belgium, 10Laboratório Especial de Micologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil, 11Universidade Federal de Goiás, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular, Goiânia, Goiás, Brazil, 12Centre of Molecular and Environmental Biology (CBMA), Biology Department, School of Sciences, University of Minho, Braga, Portugal, 13Laboratório de Genômica e Biocomplexidade Evolutiva, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil, 14Instituto de Pesquisa Clínica Evandro Chagas (IPEC) Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil, 15Facultad de Medicina, Departamento de Microbiología y Parasitología (Unidad de Micología), Universidad Nacional Autónoma de México, Ciudad de México, México, 16Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, NSW, Australia, 17School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, SA, Australia; Robinson Institute, University of Adelaide, Adelaide, SA, Australia, 18BDEEPEA4547, CIIL, Institut Pasteur de Lille, CHU de Lille, Université de Lille2, Lille, France, 19Unité de Parasitologie – Mycologie, Dynamyc Team, CHU Henri Mondor, APHP, Créteil, France, 20National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA, 21Mycology & Infectious Diseases, SA Pathology, University of Adelaide, Adelaide, SA, Australia, 22Unitat de Recerca, Hospital Joan XXIII, Institut de Investigacio Sanitaria Rovira I Virgili (IISPV), Universitat Rovira i Virgili, Tarragona, Spain.

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/65919

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Embargo date

2030-12-31

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