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A MULTI-EPITOPE PROTEIN FOR HIGH-PERFORMANCE SERODIAGNOSIS OF CHRONIC CHAGAS DISEASE IN ELISA AND LATERAL FLOW PLATFORMS
Produção científica do Laboratório de Epidemiologia e Sistemática Molecular.
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Abstract
We developed a protein to rapidly and accurately diagnose Chagas disease, a life-threatening illness identified by the WHO as a critical worldwide public health risk. Limitations in present day serological tests are complicating the current health situation and contributing to most infected persons being unaware of their condition and therefore untreated. To improve diagnostic testing, we developed an immunological mimic of the etiological agent, Trypanosoma cruzi, by combining ten pathogen-specific epitopes within the beta-barrel protein structure of Thermal Green Protein. The resulting multi-epitope protein, DxCruziV3, displayed high specificity and sensitivity as the antibody capture reagent in an ELISA platform with an analytical sensitivity that exceeds WHO recommendations. Within an immunochromatographic platform, DxCruziV3 showed excellent performance for the point of application diagnosis in a region endemic for multiple diseases, the municipality of Barcelos in the state of Amazonas, Brazil. In total, 167 individuals were rapidly tested using whole blood from a finger stick. As recommended by the Brazilian Ministry of Health, venous blood samples were laboratory tested by conventional assays for comparison. Test results suggest utilizing DxCruziV3 in different assay platforms can confidently diagnose chronic infections by T. cruzi. Rapid and more accurate results will benefit everyone but will have the most noticeable impact in resource-limited rural areas where the disease is endemic.
Publisher
MDPI
Citation
DIAS, Evandro da Rocha et al. A multi-epitope protein for high-performance serodiagnosis of chronic Chagas disease in ELISA and lateral flow platforms. International Journal of Molecular Sciences, v. 25, n. 18, p. 1-16, 11 Sept. 2024.DOI
10.3390/ijms25189811ISSN
1661-6596Notes
Produção científica do Laboratório de Doenças Parasitárias.Produção científica do Laboratório de Epidemiologia e Sistemática Molecular.
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