| Author | Abreu, Hellen | |
| Author | Royer, Carla Adriane | |
| Author | Poitevin, Carolina Gracia | |
| Author | Kohler, Ana Flávia | |
| Author | Rodrigues, Ana Carolina | |
| Author | Raboni, Sonia Mara | |
| Author | Nogueira, Meri Bordignon | |
| Author | Cardoso, Pedro Henrique | |
| Author | Arruda, Monica Barcellos | |
| Author | Baptista, Patrícia Alvarez da Silva | |
| Author | Bonatto, Ana Claudia | |
| Author | Gradia, Daniela Fiori | |
| Author | Adamoski, Douglas | |
| Author | Souza, Emanuel Maltempi de | |
| Author | Oliveira, Jaqueline Carvalho de | |
| Access date | 2024-11-21T18:52:39Z | |
| Available date | 2024-11-21T18:52:39Z | |
| Document date | 2023 | |
| Citation | ABREU, Hellen et al. Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR. Viruses, v. 15, n. 12, p. 1-10, 2024. | en_US |
| ISSN | 1999-4915 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67195 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Influenza A, like Omicron SARS-CoV-2, Is Similarly Detected in Saliva or Nasopharyngeal Samples via RT-qPCR | en_US |
| Type | Article | en_US |
| DOI | 10.3390/v15122352 | |
| Abstract | After the Coronavirus pandemic, the importance of virus surveillance was highlighted, reinforcing the constant necessity of discussing and updating the methods for collection and diagnoses, including for other respiratory viruses. Although the nasopharyngeal swab is the gold-standard sample for detecting and genotyping SARS-CoV-2 and Influenza viruses, its collection is uncomfortable and requires specialized teams, which can be costly. During the pandemic, non invasive saliva samples proved to be a suitable alternative for SARS-CoV-2 diagnosis, but for Influenza virus the use of this sample source is not recognized yet. In addition, most SARS-CoV-2 comparisons were conducted before the Omicron variant emerged. Here, we aimed to compare Influenza A and Omicron RT-qPCR analysis of nasopharyngeal swabs and saliva self-collection in paired samples from 663 individuals. We found that both nasopharyngeal swab and saliva collection are efficient for the diagnosis of Omicron (including sub-lineages) and for Influenza A, with high sensitivity and accuracy (>90%). The kappa index is 0.938 for Influenza A and 0.905 for SARS-CoV-2. These results showed excellent agreement between the two samples reinforcing saliva samples as a reliable source for detecting Omicron and highlighting saliva as a valid sample source for Influenza detection, considering this cheaper and more comfortable alternative. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Laboratório de Pesquisa em Virologia e Biologia Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Laboratório de Pesquisa em Virologia e Biologia Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Affilliation | Centro Brasileiro de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | en_US |
| Subject | Virus detection | en_US |
| Subject | Diagnostic techniques and procedures | en_US |
| Subject | Surveillance | en_US |
| Subject | Flu | en_US |
| Subject | COVID-19 | en_US |
