| Author | Gois, Luana Leandro | |
| Author | Soares, Bárbara Ribeiro | |
| Author | Silva, Carlos Gustavo Regis | |
| Author | Zanette, Dalila L. | |
| Author | Lisboa, Raphaella | |
| Author | Nascimento, Regina Santos | |
| Author | Coutinho Junior, Raimundo | |
| Author | Castro, Bernardo Galvão | |
| Author | Grassi, Maria Fernanda Rios | |
| Access date | 2024-11-28T13:12:40Z | |
| Available date | 2024-11-28T13:12:40Z | |
| Document date | 2024 | |
| Citation | GOIS, Luana Leandro et al. Imbalanced IL10/TGF-β production by regulatory T-lymphocytes in patients with HTLV-1-associated myelopathy/ tropical spastic paraparesis. BMC Infectious Diseases, v. 24, n.1, p. 1-10, 2024. | en_US |
| ISSN | 1471-2334 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67330 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Fundação Nacional de Desenvolvimento do Ensino Superior Privado (FUNADESP).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | en_US |
| Language | eng | en_US |
| Publisher | BioMed Central | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | HTLV-1 | en_US |
| Subject in Portuguese | Linfócitos T reguladores | en_US |
| Subject in Portuguese | IL10 | en_US |
| Subject in Portuguese | TGF-β | en_US |
| Subject in Portuguese | Mielopatia associada ao HTLV | en_US |
| Title | Imbalanced IL10/TGF-β production by regulatory T-lymphocytes in patients with HTLV-1-associated myelopathy/ tropical spastic paraparesis | en_US |
| Type | Article | en_US |
| DOI | 10.1186/s12879-024-09494-8 | |
| Abstract | Background: Human T-cell lymphotropic virus type 1 (HTLV-1), also denominated Human T-cell leukemia virus-1, induces immune activation and secretion of proinflammatory cytokines, especially in individuals with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Regulatory T lymphocytes (Tregs) may control of inflammation through the production of regulatory cytokines, including IL10 and TGF-β. In this study we determined the frequencies of CD4 + and CD8 + Tregs in a HAM/TSP population, compared to asymptomatic carriers and uninfected individuals, as well as investigated the profiles of regulatory and inflammatory cytokines. Methods: Asymptomatic HTLV-1 carriers and HAM/TSP patients were matched by sex and age. The frequencies of IL10- and/or TGF-β-producing Tregs were quantified by flow cytometry. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to quantify HTLV-1 proviral load and the mRNA expression of cytokines and cellular receptors in peripheral blood mononuclear cells. Results: Total frequencies of CD4 + Tregs, as well as the IL10-producing CD4 + and CD8 + Treg subsets, were statistically higher in patients with HAM/TSP compared to asymptomatic HTLV-1-infected individuals. In addition, a positive correlation was found between the frequency of CD4 + IL10 + Tregs and proviral load in the HAM/TSP patients evaluated. A positive correlation was also observed between gene expression of proinflammatory versus regulatory cytokines only in HAM / TSP group. Conclusions: A higher frequencies of IL10-producing Tregs were identified in patients with HAM/TSP. Imbalanced production of IL10 in relation to TGF-β may contribute to the increased inflammatory response characteristically seen in HAM/TSP patients. | en_US |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil. | en_US |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas a Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Affilliation | Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | en_US |
| Subject | HTLV-1 | en_US |
| Subject | Regulatory T lymphocytes | en_US |
| Subject | IL10 | en_US |
| Subject | TGF-β | en_US |
| Subject | HTLV-associated myelopathy | en_US |
| DeCS | Interleucina-10 | en_US |
| DeCS | Vírus linfotrópico T tipo 1 humano | en_US |
