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EXPLORING DENGUE INFECTION IN A VACCINATED INDIVIDUAL: PRELIMINARY MOLECULAR DIAGNOSIS AND SEQUENCING INSIGHTS.
Adelino, Talita Émile Ribeiro et al. | Date Issued: 2024
Author
Adelino, Talita Émile Ribeiro
Pedroso, Sílvia Helena Sousa Pietra
Lima, Maurício
Tomé, Luiz Marcelo Ribeiro
Guimarães, Natália Rocha
Fonseca, Vagner
Silva, Paulo Eduardo de Souza da
Moreno, Keldenn Melo Farias
Silva, Ana Cândida Araújo e
Pinheiro, Náthale Rodrigues
Souza, Carolina Senra Alves de
Alcantara, Luiz Carlos Junior
Giovanetti, Marta
Iani, Felipe Campos de Melo
Affilliation
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
Department of Exact and Earth Science. University of the State of Bahia. Salvador, BA, Brazil/Climate Amplified Diseases and Epidemics. Brasília, DF, Brazil/Centre for Epidemic Response and Innovation. School of Data Science and Computational Thinking. Stellenbosch University. Stellenbosch, South Africa
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil
Universidade Federal dos Vales do Jequitinhonha e Mucuri. Comitê Técnico Científico Multidisciplinar. Teófilo Otoni, MG, Brazil
Universidade Federal dos Vales do Jequitinhonha e Mucuri. Comitê Técnico Científico Multidisciplinar. Teófilo Otoni, MG, Brazil
Estado de Minas Gerais. Secretaria Estadual de Saúde. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/Climate Amplified Diseases and Epidemics. Brasília, DF, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/Climate Amplified Diseases and Epidemics. Brasília, DF, Brazil/Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Roma, Italy
Fundação Ezequiel Dias. Belo Horizonte, MG, Brazil
Abstract
This study examines a case involving a 7-year-old child who developed dengue symptoms following Qdenga vaccination. Despite initial negative diagnostic results, molecular analysis confirmed an infection with DENV4. Next-generation sequencing detected viral RNA from both DENV2 and DENV4 serotypes, which were identified as vaccine-derived strains using specific primers. Phylogenetic analysis further confirmed that these sequences belonged to the Qdenga vaccine rather than circulating wild-type viruses. This case underscores the critical need for precise diagnostic interpretation in vaccinated individuals to avoid misdiagnosis and to strengthen public health surveillance. A comprehensive understanding of vaccine-induced viremia is essential for refining dengue surveillance, improving diagnostic accuracy, and informing public health strategies in endemic regions.
Keywords
DENV monitoring
next-generation sequencing
genomic surveillance
vaccine breakthrough
 
Publisher
MPDI
Citation
ADELINO, Talita Émile Ribeiro et al. Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights. Viruses, v. 16, n. 10, 1603, 2024. doi.org/10.3390/v16101603
ISSN
1999-4915