Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/6754
Title: Antifungal and other Biological Activities from Oudemansiella Canarii(Basidiomycota)
Authors: Rosa, Luiz Henrique
Cota, Betania Barros
Machado, Kátia Maria Gomes
Rosa, Carlos Augusto
Zani, Carlos Leomar
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquizas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquizas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Centro Tecnologico de Minas Gerais. Belo Horizonte, MG, Brasil/ Universidade Catolica de Santos. Santos, SP, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquizas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil
Abstract: The ethyl acetate extract from the fungus Oudemansiella canarii grown in malt extract medium was evaluated against (a) the recombinant enzyme trypanothione reductase from Trypanosoma cruzi, (b) lymphocyte proliferation in human peripheral blood mononuclear cells (PBMCs) stimulated with phytohaemaglutinin, (c) the human tumour cell lineages MCF-7, TK-10 and UACC-62, and (d) the phytopathogenic fungus Cladosporium sphaerospermum. At 10 μg/ml, the crude extract was inactive against PBMC but inhibited the growth of UACC-62 cells by 47% and the enzyme trypanothione reductase (TryR). It also presented strong inhibition in the bioautographic assay with C. sphaerospermum. Chromatographic fractionation guided by this assay allowed the isolation of oudemansin A (1), a known fungitoxic compound that showed a minimum inhibitory concentration (MIC) of 1.25 μg/spot in the bioautographic assay. As oudemansin A was not active in the other assays, other components in the extract may be responsible for the observed activities by the crude extract against the UACC-62 cells or the TryR enzyme.
Keywords: Basidiomycetes
cancer cells
lymphocytes
oudemansin A
PBMC
phytopathogens
Trypanosoma cruzi
Issue Date: 2005
Publisher: Springer
Citation: ROSA, Luiz Henrique et al. Antifungal and other Biological Activities from Oudemansiella Canarii(Basidiomycota). World Journal of Microbiology and Biotechnology. October 2005, 21 (6-7): 983-987
DOI: 10.1073/pnas.0409642102
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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