| Author | Bernardi, Yanel E. | |
| Author | Sanchez-Vasquez, Estefania | |
| Author | Márquez, Rocío Belén | |
| Author | Piacentino, Michael L. | |
| Author | Urrutia, Hugo | |
| Author | Rossi, Izadora | |
| Author | Saraiva, Karina L. Alcântara | |
| Author | Pereira-Neves, Antonio | |
| Author | Ramirez, Marcel I. | |
| Author | Bronner, Marianne E. | |
| Author | Miguel, Natalia | |
| Author | Strobl-Mazzulla, Pablo H. | |
| Access date | 2024-12-14T12:44:38Z | |
| Available date | 2024-12-14T12:44:38Z | |
| Document date | 2024 | |
| Citation | BERNARDI, Yanel E. et al. miR-203 secreted in extracellular vesicles mediates the communication between neural crest and placode cells required for trigeminal ganglia formation. PLoS Biology, v. 22, n. 7 (e3002074), p. 1-21, 2024. | en_US |
| ISSN | 1545-7885 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67597 | |
| Sponsorship | This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT 2018-1879 to P.H.S-M.) and by the Fogarty International Center of the National Institutes of Health (R21TW011224 to M.E.B. and P.H.S-M.). | en_US |
| Language | eng | en_US |
| Publisher | PLOS | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Vesículas extracelulares | en_US |
| Subject in Portuguese | Comunicação intercelular | en_US |
| Subject in Portuguese | Gânglio Trigeminal | en_US |
| Title | miR-203 secreted in extracellular vesicles mediates the communication between neural crest and placode cells required for trigeminal ganglia formation | en_US |
| Type | Article | en_US |
| DOI | 10.1371/journal.pbio.3002074 | |
| Abstract | While interactions between neural crest and placode cells are: critical for the proper formation of the trigeminal ganglion, the mechanisms underlying this process remain largely uncharacterized. Here, by using chick embryos, we show that the microRNA (miR)-203, whose epigenetic repression is required for neural crest migration, is reactivated in coalescing and condensing trigeminal ganglion cells. Overexpression of miR-203 induces ectopic coalescence of neural crest cells and increases ganglion size. By employing cell-specific electroporations for either miR-203 sponging or genomic editing using CRISPR/Cas9, we elucidated that neural crest cells serve as the source, while placode cells serve as the site of action for miR-203 in trigeminal ganglion condensation. Demonstrating intercellular communication, overexpression of miR-203 in the neural crest in vitro or in vivo represses an miR responsive sensor in placode cells. Moreover, neural crest-secreted extracellular vesicles (EVs), visualized using pHluorin-CD63 vector, become incorporated into the cytoplasm of placode cells. Finally, RT-PCR analysis shows that small EVs isolated from condensing trigeminal ganglia are selectively loaded with miR-203. Together, our findings reveal a critical role in vivo for neural crest-placode communication mediated by sEVs and their selective microRNA cargo for proper trigeminal ganglion formation. | en_US |
| Affilliation | Instituto Tecnológico de Chascomús. Laboratory of Developmental Biology. Chascomús, Argentina / Escuela de Bio y Nanotecnologías. Chascomús, Argentina. | en_US |
| Affilliation | Instituto Tecnológico de Chascomús. Laboratory of Developmental Biology. Chascomús, Argentina / Escuela de Bio y Nanotecnologías. Chascomús, Argentina. | en_US |
| Affilliation | Instituto Tecnológico de Chascomús. Laboratory of Developmental Biology. Chascomús, Argentina / Escuela de Bio y Nanotecnologías. Chascomús, Argentina. | en_US |
| Affilliation | California Institute of Technology. Division of Biology. Pasadena, California, USA. | en_US |
| Affilliation | California Institute of Technology. Division of Biology. Pasadena, California, USA. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistemática de Tripanossomatídeos. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Núcleo de Plataformas Tecnológicas. Recife, PE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistemática de Tripanossomatídeos. Curitiba, PR, Brasil. | en_US |
| Affilliation | California Institute of Technology. Division of Biology. Pasadena, California, USA. | en_US |
| Affilliation | Escuela de Bio y Nanotecnologías. Chascomús, Argentina / Instituto Tecnológico Chascomús. Laboratorio de Parásitos Anaerobios. Chascomús, Argentina. | en_US |
| Affilliation | Instituto Tecnológico de Chascomús. Laboratory of Developmental Biology. Chascomús, Argentina / Escuela de Bio y Nanotecnologías. Chascomús, Argentina. | en_US |
| Subject | Trigeminal ganglion | en_US |
| Subject | microRNA | en_US |
| Subject | CRISPR/Cas9 | en_US |
| Subject | Intercellular communication | en_US |
| Subject | Extracellular vesicles | en_US |
| DeCS | Vesículas extracelulares | en_US |
| DeCS | MicroRNAs | en_US |
| DeCS | Comunicação Celular | en_US |
| DeCS | Proteína 9 Associada à CRISPR | en_US |
| DeCS | Gânglio Trigeminal | en_US |
