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CARDIAC DEVELOPMENT LONG NON-CODING RNA (CARDEL) IS ACTIVATED DURING HUMAN HEART DEVELOPMENT AND CONTRIBUTES TO CARDIAC SPECIFICATION AND HOMEOSTASIS
Pereira, Isabela T et al. | Date Issued: 2024
Author
Pereira, Isabela T
Gomes-Júnior, Rubens
Hansel-Frose, Aruana
França, Rhaíza S. V
Liu, Man
Soliman, Hossam A. N
Chan, Sunny S. K
Dudley, Samuel C Jr
Kyba, Michael
Dallagiovanna, Bruno
Affilliation
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.
University of Minnesota. Department of Medicine. Division of Cardiology. Minneapolis, USA / University of Minnesota. Lillehei Heart Institute. Minneapolis, USA.
University of Minnesota. Lillehei Heart Institute. Minneapolis, USA / University of Minnesota. Department of Pediatrics. Minneapolis, USA.
University of Minnesota. Lillehei Heart Institute. Minneapolis, USA / University of Minnesota. Department of Pediatrics. Minneapolis, USA / University of Minnesota. Stem Cell Institute. Minneapolis, USA.
University of Minnesota. Department of Medicine. Division of Cardiology. Minneapolis, USA / University of Minnesota. Lillehei Heart Institute. Minneapolis, USA.
University of Minnesota. Lillehei Heart Institute. Minneapolis, USA / University of Minnesota. Department of Pediatrics. Minneapolis, USA.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil.
Abstract
Successful heart development depends on the careful orchestration of a network of transcription factors and signaling pathways. In recent years, in vitro cardiac differentiation using human pluripotent stem cells (hPSCs) has been used to uncover the intricate gene-network regulation involved in the proper formation and function of the human heart. Here, we searched for uncharacterized cardiac-development genes by combining a temporal evaluation of human cardiac specification in vitro with an analysis of gene expression in fetal and adult heart tissue. We discovered that CARDEL (CARdiac DEvelopment Long non-coding RNA; LINC00890; SERTM2) expression coincides with the commitment to the cardiac lineage. CARDEL knockout hPSCs differentiated poorly into cardiac cells, and hPSC-derived cardiomyocytes showed faster beating rates after controlled overexpression of CARDEL during differentiation. Altogether, we provide physiological and molecular evidence that CARDEL expression contributes to sculpting the cardiac program during cell-fate commitment.
Keywords in Portuguese
Cardiomiócitos
Expressão gênica
Desenvolvimento cardíaco
 
Keywords
Cardiomyocytes
lncRNA
Gene expression
Cardiac development
 
DeCS
Homeostase
RNA Longo não Codificante
Coração
 
Publisher
MDPI
Citation
PEREIRA, I. T. et al. Cardiac development long non-coding RNA (CARDEL) is activated during human heart development and contributes to cardiac specification and homeostasis. Cells, v. 13, n. 1050, p. 1-17, 2024.
DOI
10.3390/cells13121050
ISSN
2012-9999
Notes
Data Availability Statement: RNA-Seq data generated in this study have been deposited in the NCBI Sequence Read Archive under accession number PRJNA926378 and are publicly available as of the date of publication. RNA-seq data of in vitro cardiac differentiation was previously generated by our group [17] and is available at NCBI Sequence Read Archive, accession number SRP150416. RNA-seq data heart tissues were acquired from European Nucleotide Archive (ENA) (http://www.ebi.ac.uk/ena, accessed on 25 May 2022). Accession numbers for adult heart tissue data: ERR315356, ERR315430, ERR315367 and ERR315331. Project number for fetal heart tissue data: PRJEB27811 [18].