Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/6813
Title: Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3 Þ CD16 –CD56 þNatural Killer T Cells and CD4 þ CD25 High Regulatory T Lymphocytes
Authors: Avelar, Danielle Marquete Vitelli
Avelar, Renato Sathler
Dias, João Carlos Pinto
Pascoal, Vanessa Peruhype Magalhães
Carvalho, Andréa Teixeira de
Lage, Paula Souza
Santos, Silvana Maria Elói
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Patologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Analises Clínicas. Ouro Preto, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil.
Abstract: Several studies have demonstrated that different clinical manifestations of human Chagas’ disease are associated with distinct and complex host–parasite relationships directly involving the immune system. In this context, it has been proposed that tissue damage might be more severe in the absence of regulatory mechanisms that involve both innate and adaptive immune responses. Herein, we describe a descriptive phenotypic profile focusing on the frequency of major regulatory T cells [CD4 þ CD25 high and natural killer T (NKT) lymphocytes] in different clinical forms of Chagas’ disease. Ex vivo immunophenotyping of whole blood demonstrated that the indeterminate clinical form displays a higher frequency of both CD4 þ CD25 high and NKT regulatory cells (CD3 þ CD16 – CD56 þ ), associated with increased levels of circulating cytotoxic NK cells (CD3 – CD16 þ CD56 þ and CD3 – CD16 þ CD56 dim NK cells). By con-trast, the increased percentage of activated CD8 þ HLA-DR þ T-cell subset was exclusively associated with severe clinical forms of Chagas’ disease. We hypothe-size that regulatory T cells may be able to control the deleterious cytotoxic activity in the indeterminate clinical form by inhibiting the activation of CD8 þ HLA-DR þ T cells. The lack of regulated populations in cardiac and digestive clinical forms could account for impaired immune response that culminates in strong cytotoxic activity and tissue damage.
Keywords: Chagas’ disease
Trypanosoma cruzi
Issue Date: 2005
Publisher: Blackwell Publishing
Citation: AVELAR, Danielle Marquete Vitelli et al. Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3 Þ CD16 –CD56 þNatural Killer T Cells and CD4 þ CD25 High Regulatory T Lymphocytes. Scandinavian Journal of Immunology, n. 62, p. 297-308, 2005.
DOI: doi: 10.1111/j.1365-3083.2005.01668.x
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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