| Author | Oliveira, Gabriela de | |
| Author | Costa-Rocha, Ismael Artur | |
| Author | Oliveira-Carvalho, Nani | |
| Author | Santos, Tâmilla Mayane Alves Fidelis dos | |
| Author | Campi-Azevedo, Ana Carolina | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Miranda, Vitor Hugo Simões | |
| Author | Prado, Roberta Oliveira | |
| Author | Pereira, Agnes Antônia Sampaio | |
| Author | Alves, Clarice Carvalho | |
| Author | Brito-de-Sousa, Joaquim Pedro | |
| Author | Reis, Laise Rodrigues | |
| Author | Costa-Pereira, Christiane | |
| Author | Mata, Camila Pacheco Silveira Martins da | |
| Author | Almeida, Vanessa Egídio Silveira | |
| Author | Santos, Liliane Martins dos | |
| Author | Almeida, Gregório Guilherme | |
| Author | Antonelli, Lis Ribeiro do Valle | |
| Author | Coelho-dos-Reis, Jordana Grazziela | |
| Author | Carvalho, Andréa Teixeira | |
| Access date | 2025-01-23T19:41:52Z | |
| Available date | 2025-01-23T19:41:52Z | |
| Document date | 2024 | |
| Citation | DE OLIVEIRA, Gabriela et al. Phenotypic Timeline Kinetics, Integrative Networks, and Performance of T- and BCell Subsets Associated with Distinct Clinical Outcome of Severe COVID-19 Patients. Microorganisms, v. 12, n. 11, 2272, 2024. | en_US |
| ISSN | 2076-2607 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68222 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | COVID-19 | en_US |
| Title | Phenotypic Timeline Kinetics, Integrative Networks, and Performance of T- and BCell Subsets Associated with Distinct Clinical Outcome of Severe COVID-19 Patients. | en_US |
| Type | Article | en_US |
| DOI | 10.3390/microorganisms12112272 | |
| Abstract | The present study aimed to evaluate the kinetics of the phenotypic profile and integrative networks of T/B-cells in severe COVID-19 patients, categorized according to disease outcome, during the circulation of the B.1.1.28 and B.1.1.33 SARS-CoV-2 strains in Brazil. Peripheral blood obtained at distinct time points (baseline/D0; D7; D14-28) was used for ex vivo flow cytometry immunophenotyping. The data demonstrated a decrease at D0 in the frequency of CD3+ T-cells and CD4+ T-cells and an increase in B-cells with mixed activation/exhaustion profiles. Higher changes in B-cell and CD4+ T-cells at D7 were associated with discharge/death outcomes, respectively. Regardless of the lower T/B-cell connectivity at D0, distinct profiles from D7/D14-28 revealed that, while discharge was associated with increasing connectivity for B-cells, CD4+ and CD8+ T-cells death was related to increased connectivity involving B-cells, but with lower connections mediated by CD4+ T-cells. The CD4+CD38+ and CD8+CD69+ subsets accurately classified COVID-19 vs. healthy controls throughout the kinetic analysis. Binary logistic regression identified CD4+CD107a+, CD4+T-bet+, CD8+CD69+, and CD8+T-bet+ at D0 and CD4+CD45RO+CD27+ at D7 as subsets associated with disease outcomes. Results showed that distinct phenotypic timeline kinetics and integrative networks of T/B-cells are associated with COVID-19 outcomes that may subsidize the establishment of applicable biomarkers for clinical/therapeutic monitoring. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Uberlândia. Departamento de Imunologia e Parasitologia. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Hospital Risoleta Tolentino Neves. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Hospital Risoleta Tolentino Neves. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | en_US |
| Subject | COVID-19 | en_US |
| Subject | disease outcome | en_US |
| Subject | cellular memory | en_US |
| Subject | activation | en_US |
| Subject | exhaustion | en_US |
