Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/6854
Title: Influence of parasite presence on the immunologic profile of peripheral blood mononuclear cells from chagasic patients after specific drug therapy
Authors: Dutra, Walderez Ornelas
Luz, Zélia Maria Profeta da
Cançado, Joaquim Romeu
Pereira, Maria Elisabeth Soares
Nunes, Rosa Maria Brito
Galvão, Lúcia Maria da Cunha
Cooley, Daniel G.
Brener, Zigman
Gazzinelli, Giovanni
Parra, Jucara Farhat de Carvalho
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Hospital das Clínicas. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
United States Public Health Service. National Center for Infectious Diseases. Center for Diseases Control and Prevention. Division of Parasitic Diseases. Department of Health and Human Services. Atlanta, GA, USA.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Abstract: The aim of this study is to evaluate the effects of parasite clearance on the immunological profile of peripheral blood mononuclear cells (PBMC) from chagasic patients submitted to specific drug therapy. PBMC were examined by flow cytometry and proliferative responsiveness to Trypanosoma cruzi-related stimuli. Three groups of patients were studied: not treated (NT), treated not cured (TNC) and cured (C). All data were compared to values from uninfected individuals (NI). NT displayed a lower percentage of CD3+ cells as compared to NI, while TNC and C had mean values that were between those from NI and NT. Infected patients had double the percent of CD3+ HLA-DR+ cells, independent of the efficacy of the treatment. Thus, absence of circulating parasites did not reduce T cell activation in Chagas' disease. NT displayed a higher percentage of CD5+ B cells as compared to NI, while TNC and C had mean values between those from NI and NT. In contrast to the phenotypic data, the in vitro mean proliferative responses to parasite-related stimuli of PBMC from C were reduced to the low mean levels observed in NI. These striking differences were statistically different from the high responses seen in NT and TNC. Our data suggest that proliferative responses of PBMC from C reflect immunological changes due elimination of parasite
Keywords: Antigens, CD5/metabolism
Antigens, Protozoan
Chagas Disease/drug therapy
Chagas Disease/parasitology
Leukocytes, Mononuclear/immunology
T-Lymphocytes/immunology
Issue Date: 1996
Publisher: Wiley Online Library
Citation: DUTRA, Walderez Ornelas et al. Influence of parasite presence on the immunologic profile of peripheral blood mononuclear cells from chagasic patients after specific drug therapy. Parasite Immunology, v. 18, n. 11, p. 579-585, Nov. 1996.
ISSN: 1365-3024
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Walderez_Dutra_etal_IRR_1996.pdf313.23 kBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.