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https://www.arca.fiocruz.br/handle/icict/68738
MORPHO-PHYSIOLOGICAL STUDIES ON IN VITRO GERMINATION OF BERBERIS EMPETRIFOLIA LAM. (BERBERIDACEAE)
Gibberellic acid
Medicinal plant
Post-seminal development
Pre-germinative treatments
Author
Affilliation
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Universidade de La Frontera. Faculty of Medicine. Department of Basic Sciences. Temuco, Chile.
Universidade de La Frontera. Faculty of Medicine. Department of Basic Sciences. Temuco, Chile.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Universidade de La Frontera. Faculty of Medicine. Department of Basic Sciences. Temuco, Chile.
Universidade de La Frontera. Faculty of Medicine. Department of Basic Sciences. Temuco, Chile.
Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcantara Gomes. Laboratório de Biotecnologia de Plantas. Rio de Janeiro, RJ, Brasil.
Abstract
Berberis empetrifolia is an endemic species from Chile that produces some bioactive compounds, highlighting the alkaloid berberine, which demonstrated antitumor properties. Taking into account the constant plant supply demand required for medicinal use, the establishment of in vitro propagation protocols allows plant multiplication, without affecting natural populations. Moreover, the in vitro germination is an efficient strategy to obtain axenic plants. The aim of this study was to establish the in vitro germination and to characterize seeds morphology and seedling development of B. empetrifolia. After decontamination of seeds, pre-germinative treatments were applied. Seed viability ranged from 75-90% and the embedding curve revealed permeable integument. The germination was characterized as epigeal phanerocotylar type. Seeds not submitted to pre-germinative treatments reached 10-30% germination rate (GR) and showed asynchronous germination. The most efficient pre-germinative treatment was the storage of seeds at 3˚C for 12 days followed by overnight soaking in GA3 solution associated to seed coat removal. This treatment allowed the seed dormancy to be overcome, reaching GR above 95% and resulting in synchronous germination. The in vitro germination protocol established for B.empetrifolia was efficient to produce phenotypically normal axenic seedlings, allowing their use as source of plant material for biotechnological, phytochemical and pharmacological studies.
Keywords
DormancyGibberellic acid
Medicinal plant
Post-seminal development
Pre-germinative treatments
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