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https://www.arca.fiocruz.br/handle/icict/69285
SEQUENCING AND ANALYSIS OF WOLBACHIA STRAINS FROM A AND B SUPERGROUPS DETECTED IN SYLVATIC MOSQUITOES FROM BRAZIL
Author
Affilliation
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Departamento de Entomologia, Recife 21040-900, PE, Brasil.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Núcleo de Ciências da Vida, Centro Acadêmico do Agreste, Universidade Federal de Pernambuco (UFPE), Caruaru 50670-901, PE, Brazil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Department of Arbovirology and Entomology, Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Center for Arbovirus and Hemorrhagic Fever Reference and Research, National Reference Center for Tropical Infectious Diseases, Bernhard-Nocht-Straße, 74, 20359 Hamburg, Germany.
Universidade Federal Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Departamento de Entomologia, Recife 21040-900, PE, Brasil.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAIC-SM), College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Núcleo de Ciências da Vida, Centro Acadêmico do Agreste, Universidade Federal de Pernambuco (UFPE), Caruaru 50670-901, PE, Brazil.
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Núcleo de Bioinformática e Departamento de Entomologia, Recife 21040-900, PE, Brasil.
Department of Arbovirology and Entomology, Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Center for Arbovirus and Hemorrhagic Fever Reference and Research, National Reference Center for Tropical Infectious Diseases, Bernhard-Nocht-Straße, 74, 20359 Hamburg, Germany.
Universidade Federal Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil.
Abstract
Wolbachia are endosymbiotic bacteria that infect a wide range of arthropods and filarial nematodes, often manipulating host reproduction. The efficacy of Wolbachia-based interventions for dengue and chikungunya control has been validated through numerous field studies in recent years. This study aimed to investigate the diversity and prevalence of Wolbachia infections in sylvatic mosquitoes from two locations in Recife, Brazil. Multiple mosquito species were screened for Wolbachia using both target marker gene amplification coupled with Sanger sequencing and whole-genome sequencing (WGS) approaches. Phylogenetic analyses were conducted to classify Wolbachia strains into supergroups and assess their evolutionary relationships. Results revealed the presence of Wolbachia in eleven mosquito species examined, with different infection rates. Both supergroups A and B of Wolbachia strains were identified, with Aedes albopictus showing co-infection by both supergroups through the WGS approach. We also detected indirect evidence of Wolbachia horizontal transmission among mosquitoes and other distant host orders. This study provides valuable insights into the distribution and diversity of Wolbachia in sylvatic mosquitoes from Brazil and adds new important data about Wolbachia detection through target marker gene amplicon coupled with Sanger sequencing and WGS methods, highlighting its complementarity to ascertain the presence of Wolbachia in mosquito samples.
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