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Sustainable Development Goals
03 Saúde e Bem-Estar09 Indústria, inovação e infraestrutura
10 Redução das desigualdades
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POLYMORPHISMS IN THE ACE I/D (RS4646994) AND ACE2 G8790A (RS2285666) IN YOUNG CHILDREN LIVING IN THE AMAZON REGION AND SARS-COV-2 INFECTION
Institutional Review Board Statement: This study is currently approved by the Ethics Committee of the “Universidade Federal de Roraima” (UFRR), Brazil (Approval number: CAAE 45542515.1.0000.5302, on 23 November 2015), and was conducted according to the guidelines of the Declaration of Helsinki. Fecal samples were manipulated anonymously, and patients’ data were maintained securely.
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COVID-19 infection caused by SARS-CoV-2 continues to cause significant mortality and morbidity. ACE2 is a key regulator of the renin–angiotensin–aldosterone system (RAAS). Differences in COVID-19 severity are thought to be due to the imbalance of RAAS/ACE mutations. This retrospective study evaluated the detection and genetic susceptibility to SARS-CoV-2 infection in 202 children ≤3 years of age living in the Amazon region in 2021. The angiotensin-converting enzyme ACE I/D (rs4646994) and ACE2 G8790A (rs2285666) polymorphisms were detected by SYBR GREEN real-time PCR and PCR-RFLP/Alul digestion, respectively. SARS-CoV-2 detection was performed by RT-qPCR in feces and saliva samples collected simultaneously from the same children presenting acute gastroenteritis (AGE) or acute respiratory infection (ARI). The frequency of SARS-CoV-2 detected by qRT-PCR in children was low (5.9%, 12/202), although higher in the group of children with AGE (8.9%, 9/101) than with ARI (2.9%, 3/101). Susceptibility to SARS-CoV-2 infection was not verified due to the low frequency. Homozygous II (rs4646994) children were the majority (87.1%, 176/202). Boys with genotype A (rs2285666) were more susceptible to ARI and pneumonia symptoms than AGE (OR = 3.8, 95% CI 1.4–10.3, p 0.007). Boys with genotype G (rs4646994) or the combination II + G were more susceptible to acquiring AGE. Surveillance, along with understanding their causes, is crucial to controlling ARI and COVID-19 in children living in low-income countries.
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MDPI
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PIMENTA, Yan Cardoso et al. Polymorphisms in the ACE I/D (rs4646994) and ACE2 G8790A (rs2285666) in young children living in the Amazon Region and SARS-CoV-2 infection. Tropical Medicine and Infectious Disease, v. 9, n. 11, p. 1-13, 7 Nov. 2024.DOI
10.3390/tropicalmed9110270ISSN
2414-6366Notes
Produção científica do Laboratório de Virologia Comparada e Ambiental.Institutional Review Board Statement: This study is currently approved by the Ethics Committee of the “Universidade Federal de Roraima” (UFRR), Brazil (Approval number: CAAE 45542515.1.0000.5302, on 23 November 2015), and was conducted according to the guidelines of the Declaration of Helsinki. Fecal samples were manipulated anonymously, and patients’ data were maintained securely.
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