| Author | Bahia, Diana | |
| Author | Avelar, Lívia das Gracas Amaral | |
| Author | Mortara, Renato Arruda | |
| Author | Khayath, Naji | |
| Author | Noël, Christophe | |
| Author | Capron, Monique | |
| Author | Dissous, Colette | |
| Author | Pierce, Raymond John | |
| Author | Oliveira, Guilherme Corrêa de | |
| Access date | 2013-10-09T18:23:37Z | |
| Available date | 2013-10-09T18:23:37Z | |
| Document date | 2006 | |
| Citation | BAHIA, Diana et al.SmPKC1, a new protein kinase C identified in the platyhelminth parasiteSchistosoma mansoni. Biochem. biophys. res. commun. 2006; 345(3):1138–1148 | pt_BR |
| ISSN | 0006-291X | |
| URI | https://www.arca.fiocruz.br/handle/icict/7104 | |
| Language | eng | pt_BR |
| Publisher | Academic Press | pt_BR |
| Rights | restricted access | pt_BR |
| Title | SmPKC1, a new protein kinase C identified in the platyhelminth parasiteSchistosoma mansoni | pt_BR |
| Type | Article | pt_BR |
| Abstract | Schistosoma mansoni signal transduction pathways are promising sources of target molecules for the development of novel control strategies against this platyhelminth parasite of humans. Members of the protein kinase C (PKC) family play key roles in such pathways activated by both receptor tyrosine kinases and other receptors, controlling a variety of physiological processes. Here, we report the cloning and molecular characterization of the first PKC identified in S. mansoni. Structural analysis indicated that SmPKC1 exhibits all the features typical of the conventional PKC subfamily. The gene structure was determined in silico and found to comprise a total of 15 exons and 14 introns. This structure is highly conserved; all intron positions are also present in the human PKCβ gene and most of the exon sizes are identical. Using PCR on genomic DNA we were able to show that putative orthologues of SmPKC1 are present in 9 Schistosoma species. SmPKC1 expression is developmentally regulated with the highest level of transcripts in miracidia, whereas SmPKC1 protein expression is higher in the sporocyst. The localization of SmPKC1 on the sporocyst ridge cyton and in schistosomula acetabular glands suggests that the enzyme plays a role in signal transduction pathways associated with larval transformation. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Programa de Pòs-Graduação e Pesquisa. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Institut Pasteur de Lille. Lille, France | pt_BR |
| Affilliation | University of Newcastle upon Tyne. School of Biology. Institute for Research on Environment and Sustainability. Devonshire Building, UK | pt_BR |
| Affilliation | Institut Pasteur de Lille. Lille, France | pt_BR |
| Affilliation | Institut Pasteur de Lille. Lille, France | pt_BR |
| Affilliation | Institut Pasteur de Lille. Lille, France | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Programa de Pòs-Graduação e Pesquisa. Belo Horizonte, Brazil | pt_BR |
| Subject | Protein kinase C | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Parasite | pt_BR |
| Subject | Signal transduction | pt_BR |
| Subject | Gene | pt_BR |
