Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7104
Title: SmPKC1, a new protein kinase C identified in the platyhelminth parasiteSchistosoma mansoni
Authors: Bahia, Diana
Avelar, Lívia das Gracas Amaral
Mortara, Renato Arruda
Khayath, Naji
Noël, Christophe
Capron, Monique
Dissous, Colette
Pierce, Raymond J.
Oliveira, Guilherme Corrêa de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Programa de Pòs-Graduação e Pesquisa. Belo Horizonte, MG, Brazil
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil
Institut Pasteur de Lille. Lille, France
University of Newcastle upon Tyne. School of Biology. Institute for Research on Environment and Sustainability. Devonshire Building, UK
Institut Pasteur de Lille. Lille, France
Institut Pasteur de Lille. Lille, France
Institut Pasteur de Lille. Lille, France
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Programa de Pòs-Graduação e Pesquisa. Belo Horizonte, Brazil
Abstract: Schistosoma mansoni signal transduction pathways are promising sources of target molecules for the development of novel control strategies against this platyhelminth parasite of humans. Members of the protein kinase C (PKC) family play key roles in such pathways activated by both receptor tyrosine kinases and other receptors, controlling a variety of physiological processes. Here, we report the cloning and molecular characterization of the first PKC identified in S. mansoni. Structural analysis indicated that SmPKC1 exhibits all the features typical of the conventional PKC subfamily. The gene structure was determined in silico and found to comprise a total of 15 exons and 14 introns. This structure is highly conserved; all intron positions are also present in the human PKCβ gene and most of the exon sizes are identical. Using PCR on genomic DNA we were able to show that putative orthologues of SmPKC1 are present in 9 Schistosoma species. SmPKC1 expression is developmentally regulated with the highest level of transcripts in miracidia, whereas SmPKC1 protein expression is higher in the sporocyst. The localization of SmPKC1 on the sporocyst ridge cyton and in schistosomula acetabular glands suggests that the enzyme plays a role in signal transduction pathways associated with larval transformation.
Keywords: Protein kinase C
Schistosoma mansoni
Parasite
Signal transduction
Gene
Issue Date: 2006
Publisher: Academic Press
Citation: BAHIA, Diana et al.SmPKC1, a new protein kinase C identified in the platyhelminth parasiteSchistosoma mansoni. Biochem. biophys. res. commun. 2006; 345(3):1138–1148
ISSN: 0006-291X
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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