| Author | Fonseca, Cristina Toscano | |
| Author | Pacífico, Lucila Grossi Gonçalves | |
| Author | Barsante, Michele Mendes | |
| Author | Rassi, Tatiana | |
| Author | Cassali, Geovanni Dantas | |
| Author | Oliveira, Sérgio Costa | |
| Access date | 2013-10-14T14:08:18Z | |
| Available date | 2013-10-14T14:08:18Z | |
| Document date | 2006 | |
| Citation | FONSECA, Cristina Toscano. Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone. Microbes Infect. 2006 Aug;8(9-10):2509-16. | pt_BR |
| ISSN | 1286-4579 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7135 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone | pt_BR |
| Type | Article | |
| Abstract | Schistosomiasis is an endemic disease that affects 200 million people worldwide. DNA-based vaccine is a promising strategy to induce protective immunity against schistosomiasis, since both humoral and cellular immune responses are involved in parasite elimination. In this study, we evaluated the ability of Sm14 cDNA alone or in association with a plasmid expressing murine interleukin (IL)-12 to induce protection against challenge infection. Mice were immunized with four doses of the DNA vaccine and the levels of protection were determined by worm burden recovery after challenge infection. Specific antibody production to rSm14 was determined by ELISA, and cytokine production was measured in splenocyte culture supernatants stimulated with rSm14 and in bronchoalveolar lavage of vaccinated mice after challenge infection. DNA im-munization with pCI/Sm14 alone induced 40.5% of worm reduction. However, the use of pCI/IL-12 as adjuvant to pCI/Sm14 immunization failed to enhance protection against challenge infection. Protection induced by pCI/Sm14 immunization correlates with specific IgG antibody production against Sm14, Th1 type of immune response with high levels of interferon (IFN)-gand low levels of IL-4 in splenocyte culture supernatants and in brochoalveolar lavage after challenge infection. IL-12 co-administration with pCI/Sm14 induced a significant production of nitric oxide in sple-nocyte culture supernatants and also lymphocyte suppression, with reduced percentage of T cells producing IFN-gand tumor necrosis factor-a | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Patologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Sm14 | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | DNA vaccine | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
