Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7135
Title: Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone
Authors: Fonseca, Cristina Toscano
Pacífico, Lucila Grossi Gonçalves
Barsante, Michele Mendes
Rassi, Tatiana
Cassali, Geovanni Dantas
Oliveira, Sérgio Costa
Affilliation: Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Patologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto do Milenio. Instituto de Investigação em Imunologia. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil
Abstract: Schistosomiasis is an endemic disease that affects 200 million people worldwide. DNA-based vaccine is a promising strategy to induce protective immunity against schistosomiasis, since both humoral and cellular immune responses are involved in parasite elimination. In this study, we evaluated the ability of Sm14 cDNA alone or in association with a plasmid expressing murine interleukin (IL)-12 to induce protection against challenge infection. Mice were immunized with four doses of the DNA vaccine and the levels of protection were determined by worm burden recovery after challenge infection. Specific antibody production to rSm14 was determined by ELISA, and cytokine production was measured in splenocyte culture supernatants stimulated with rSm14 and in bronchoalveolar lavage of vaccinated mice after challenge infection. DNA im-munization with pCI/Sm14 alone induced 40.5% of worm reduction. However, the use of pCI/IL-12 as adjuvant to pCI/Sm14 immunization failed to enhance protection against challenge infection. Protection induced by pCI/Sm14 immunization correlates with specific IgG antibody production against Sm14, Th1 type of immune response with high levels of interferon (IFN)-gand low levels of IL-4 in splenocyte culture supernatants and in brochoalveolar lavage after challenge infection. IL-12 co-administration with pCI/Sm14 induced a significant production of nitric oxide in sple-nocyte culture supernatants and also lymphocyte suppression, with reduced percentage of T cells producing IFN-gand tumor necrosis factor-a
Keywords: Sm14
Schistosoma mansoni
DNA vaccine
Issue Date: 2006
Publisher: Elsevier
Citation: FONSECA, Cristina Toscano. Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone. Microbes Infect. 2006 Aug;8(9-10):2509-16.
ISSN: 1286-4579
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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