| Author | Marques, Marcos José | |
| Author | Volpini, Angela Cristina | |
| Author | Coelho, George Luiz Lins Machado | |
| Author | Pinto, Jackson Machado | |
| Author | Costa, Carlos A. da | |
| Author | Mayrink, Wilson | |
| Author | Genaro, Odair | |
| Author | Romanha, Álvaro José | |
| Access date | 2013-10-15T13:41:23Z | |
| Available date | 2013-10-15T13:41:23Z | |
| Document date | 2006 | |
| Citation | MARQUES, Marcos Jose et al. Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2006 Jan;54(1):37-43. | pt_BR |
| ISSN | 0732-8893 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7147 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Biomedical | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction | pt_BR |
| Type | Article | pt_BR |
| Abstract | An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PCR) was similar to that of immunofluorescence assay and enzyme-linked immunosorbent assay, and higher than that of MST and PE (P < .05). In the group of patients suspected to have ACL, PCR presented the same positivity as PE and MST together. No correlation between positivity of the laboratory methods and clinical or epidemiologic aspects was observed. Our data confirmed the value of PCR as an alternative laboratory method for diagnosing ACL, especially for those patients with negative PE and MST. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Pesquisas em Leishmanioses. Rio de Janeiro, RJ, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Escola de Farmacia. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Clınica Dermatologica da Santa Casa de Misericordia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | American cutaneous leishmaniasis | pt_BR |
| Subject | Polymerase chain reaction | pt_BR |
