Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7147
Title: Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction
Authors: Marques, Marcos José
Volpini, Angela Cristina
Coelho, George Luiz Lins Machado
Pinto, Jackson Machado
Costa, Carlos A. da
Mayrink, Wilson
Genaro, Odair
Romanha, Álvaro José
Affilliation: Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Pesquisas em Leishmanioses. Rio de Janeiro, RJ, Brazil
Universidade Federal de Ouro Preto. Escola de Farmacia. Ouro Preto, MG, Brazil
Clınica Dermatologica da Santa Casa de Misericordia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil
Abstract: An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PCR) was similar to that of immunofluorescence assay and enzyme-linked immunosorbent assay, and higher than that of MST and PE (P < .05). In the group of patients suspected to have ACL, PCR presented the same positivity as PE and MST together. No correlation between positivity of the laboratory methods and clinical or epidemiologic aspects was observed. Our data confirmed the value of PCR as an alternative laboratory method for diagnosing ACL, especially for those patients with negative PE and MST.
Keywords: American cutaneous leishmaniasis
Polymerase chain reaction
Issue Date: 2006
Publisher: Elsevier Biomedical
Citation: MARQUES, Marcos Jose et al. Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2006 Jan;54(1):37-43.
ISSN: 0732-8893
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
69.pdf185.46 kBAdobe PDFThumbnail
    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.