Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7181
Title: Responses of the surface membrane and excretory system of Schistosoma mansoni to damage and to treatment with praziquantel and other biomolecules
Authors: Oliveira, Fabricia Alvisi de
Kusel, John Robert
Ribeiro, Fabio
Coelho, Paulo Marcos Zach
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG,Brasil
Glasgow University. IBLS. Glasgow, Scotland
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil
Santa Casa de Misericordia. Belo Horizonte, MG, Brasil
Abstract: Damage to the surface membrane of adult Schistosoma mansoni, and the activity of the excretory system, as shown by resorufin fluorescence, was observed following treatment with praziquantel and incubation with other molecules. Praziquantel treatment induced damage to the surface membrane as measured by the use of a variety of fluorescent compounds. The excretory system of the male worm was inhibited immediately after praziquantel treatment, but fully recovered after culture for 2 h following removal of praziquantel. The excretory system of the female, observed to be minimally active in untreated worm pairs, was often greatly activated in paired females, as shown by intense resorufin labelling, after praziquantel treatment, and this continued during recovery of the male excretory system. In experiments with normal worm pairs, the female could be activated by inhibiting the metabolic rate of the pair by a cooling procedure. The effects on the excretory system of changes in culture conditions (such as changes in pH, concentrations of bacterial lipopolysaccharide, cytokines, reactive oxygen species, compounds which remove cholesterol, such as beta-methyl cyclodextrin, and damaging basic poly-L-lysine) were also assessed. It is concluded that the extensive excretory system of the adult worm is responsive to drug treatment and to certain changes in environmental conditions. Its activity seems to be strongly linked to the integrity of the surface membrane
Keywords: excretory system
Schistosoma mansoni
praziquantel
P-glycoprotein
cholesterol
beta-methyl cyclodextrin
poly-L-lysine
tumour necrosis factor-alpha
Issue Date: 2006
Publisher: Cambridge University Press
Citation: OLIVEIRA, Fabricia Alvisi de et al. Responses of the surface membrane and excretory system of Schistosoma mansoni to damage and to treatment with praziquantel and other biomolecules. Parasitology. 2006 Mar;132(Pt 3):321-30.
DOI: 10.1017/S0031182005009169
ISSN: 0031-1820
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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