| Author | Lima, Flávia Oliveira de | |
| Author | Alves, Vivian | |
| Author | Barbosa Filho, José Maria | |
| Author | Almeida, Jackson Roberto Guedes da Silva | |
| Author | Rodrigues, Luis Cezar | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Villarreal, Cristiane Flora | |
| Access date | 2013-10-25T18:58:31Z | |
| Available date | 2013-10-25T18:58:31Z | |
| Document date | 2012 | |
| Citation | LIMA,F. O. et al. Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition. Phytotherapy Research, v. 27, p. 1557–1563, 2013. | pt_BR |
| ISSN | 10.1002/ptr.4902 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7210 | |
| Language | eng | pt_BR |
| Publisher | John Wiley & Sons, Ltd | pt_BR |
| Rights | open access | pt_BR |
| Title | Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition | pt_BR |
| Type | Article | pt_BR |
| Abstract | The present study investigates the antinociceptive properties of lupeol inmodels of inflammatory and post-operative
pain, as well as its mechanisms of action. The effects of lupeol were tested against acetic acid-induced writhing,
formalin test, carrageenan-induced hyperalgesia, and post-operative pain model. Cytokine levels were determined
by ELISA. Mice motor performance was evaluated in the rota rod and open-field tests. Pre-treatment of mice with
lupeol (5–100mg/kg IP) produced a dose-related inhibition of writhing in mice. The maximal antinociception
produced by lupeol (60mg/kg) was unaffected in mice pre-treated with yohimbine (a2 adrenoceptor antagonist;
2mg/kg IP), L-arginine (substrate for nitric oxide synthase; 600mg/kg IP), glibenclamide (the KATP-channel
blocker; 2mg/kg IP), and methysergide maleate (serotoninergic receptors antagonist; 5mg/kg IP). Furthermore,
lupeol (25–100mg/kg) inhibited the late phase of formalin test. Pre-treatment with lupeol (50 and 100mg/kg)
inhibited the hyperalgesia and the local increase in tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b)
levels induced by carrageenan. In contrast, lupeol did not inhibit the post-operative pain. Lupeol-treated mice did
not show any motor performance alterations or apparent systemic toxicity. Our results demonstrate that lupeol
has consistent antinociceptive properties during inflammatory pain, but not post-operative pain, acting through
the inhibition of IL-1b and TNF-a production. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil | pt_BR |
| Subject | Lonchocarpus araripensis | pt_BR |
| Subject | Lupeol | pt_BR |
| Subject | Antinociception | pt_BR |
| Subject | Inflammatory pain | pt_BR |
| Subject | Post-operative pain | pt_BR |
| Subject | Cytokines | pt_BR |
