Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7210
Title: Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition
Authors: Lima, Flávia Oliveira de
Alves, Vivian
Barbosa Filho, José Maria
Almeida, Jackson Roberto Guedes da Silva
Rodrigues, Luis Cezar
Soares, Milena Botelho Pereira
Villarreal, Cristiane Flora
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil
Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil
Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil
Abstract: The present study investigates the antinociceptive properties of lupeol inmodels of inflammatory and post-operative pain, as well as its mechanisms of action. The effects of lupeol were tested against acetic acid-induced writhing, formalin test, carrageenan-induced hyperalgesia, and post-operative pain model. Cytokine levels were determined by ELISA. Mice motor performance was evaluated in the rota rod and open-field tests. Pre-treatment of mice with lupeol (5–100mg/kg IP) produced a dose-related inhibition of writhing in mice. The maximal antinociception produced by lupeol (60mg/kg) was unaffected in mice pre-treated with yohimbine (a2 adrenoceptor antagonist; 2mg/kg IP), L-arginine (substrate for nitric oxide synthase; 600mg/kg IP), glibenclamide (the KATP-channel blocker; 2mg/kg IP), and methysergide maleate (serotoninergic receptors antagonist; 5mg/kg IP). Furthermore, lupeol (25–100mg/kg) inhibited the late phase of formalin test. Pre-treatment with lupeol (50 and 100mg/kg) inhibited the hyperalgesia and the local increase in tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b) levels induced by carrageenan. In contrast, lupeol did not inhibit the post-operative pain. Lupeol-treated mice did not show any motor performance alterations or apparent systemic toxicity. Our results demonstrate that lupeol has consistent antinociceptive properties during inflammatory pain, but not post-operative pain, acting through the inhibition of IL-1b and TNF-a production.
Keywords: Lonchocarpus araripensis
Lupeol
Antinociception
Inflammatory pain
Post-operative pain
Cytokines
Issue Date: 2012
Publisher: John Wiley & Sons, Ltd
Citation: LIMA,F. O. et al. Antinociceptive effect of lupeol: evidence for a role of cytokines inhibition. Phytotherapy Research, v. 27, p. 1557–1563, 2013.
ISSN: 10.1002/ptr.4902
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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