| Author | Santos, Diego Moura | |
| Author | Carneiro, Marcia Weber | |
| Author | Moura, Tatiana Rodrigues de | |
| Author | Soto, Manuel | |
| Author | Luz, Nívea Farias | |
| Author | Prates, Deboraci Brito | |
| Author | Irache, Juan Manuel | |
| Author | Brodskyn, Claudia Ida | |
| Author | Barral, Aldina Maria Prado | |
| Author | Barral Netto, Manoel | |
| Author | Espuelas, Socorro | |
| Author | Borges, Valeria de Matos | |
| Author | Oliveira, Camila Indiani de | |
| Access date | 2013-11-26T14:04:47Z | |
| Available date | 2013-11-26T14:04:47Z | |
| Document date | 2013 | |
| Citation | SANTOS, D. M. et al. PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania. Nanomedicine: Nanotechnology, Biology, and Medicine, v. 9, p. 985–995, 2013. | pt_BR |
| ISSN | 1549-9634 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7262 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.nano.2013.04.003 | |
| Abstract | We recently demonstrated that immunization with polyester poly(lactide-co-glycolide acid) (PLGA) nanoparticles loaded with the 11-kDa
Leishmania vaccine candidate kinetoplastid membrane protein 11 (KMP-11) significantly reduced parasite load in vivo. Presently, we
explored the ability of the recombinant PLGA nanoparticles to stimulate innate responses in macrophages and the outcome of infection with
Leishmania braziliensis in vitro. Incubation of macrophages with KMP-11-loaded PLGA nanoparticles significantly decreased parasite load.
In parallel, we observed the augmented production of nitric oxide, superoxide, TNF-α and IL-6. An increased release of CCL2/MCP-1 and
CXCL1/KC was also observed, resulting in macrophage and neutrophil recruitment in vitro. Lastly, the incubation of macrophages with
KMP-11-loaded PLGA nanoparticles triggered the activation of caspase-1 and the secretion of IL-1β and IL-18, suggesting inflammasome
participation. Inhibition of caspase-1 significantly increased the parasite load. We conclude that KMP-11-loaded PLGA nanoparticles
promote the killing of intracellular Leishmania parasites through the induction of potent innate responses. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidad Autonoma de Madrid. Centro de Biología Molecular Severo Ochoa. Departamento de Biologia Molecular. Madrid, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidad de Navarra. Instituto de Salud Tropical. Pamplona, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil | pt_BR |
| Affilliation | Universidad de Navarra. Instituto de Salud Tropical. Pamplona, Spain / Universidad de Navarra. Facultad de Farmacia. Departamento de Farmacia y Tecnología Farmacéutica. Pamplona, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | PLGA | pt_BR |
| Subject | Nanoparticle | pt_BR |
| Subject | Macrophage | pt_BR |
| Subject | Innate response | pt_BR |
